IL-22和IL-2在四甲基哌啶减轻三氯乙烯致敏小鼠肝损伤中的表达

Expression of IL-22 and IL-2 in tetramethylpiperidine attenuating liver injury in mice sensitized by trichloroethylene

目的探讨腹腔注射四甲基哌啶(Tempol)对三氯乙烯(TCE)致敏小鼠肝损伤中的白细胞介素(IL)-22、IL-2及丙二醛(MDA)水平的影响。方法40只6~8周龄Balb/c雌性小鼠随机抽样分成空白组、溶剂组、TCE组和TCE+Tempol组,建立TCE经皮致敏模型,TCE+Tempol组动物在末次激发前腹腔注射Tempol溶液(100 mg/kg)。末次激发24 h后根据皮肤致敏结果评分将动物分成致敏阳性组或致敏阴性组,HE染色法观测肝脏切片病理,分离血清检测谷草转氨酶(AST)与谷丙转氨酶(ALT),TBA法检测肝脏MDA含量,IHC和Western blot法检测IL-22和IL-2在肝组织的表达水平。结果TCE组和TCE+Tempol组致敏率分别为40.0%和33.3%;病理检测结果显示:空白组、溶剂组与致敏阴性组小鼠肝细胞无明显病理变化,TCE致敏阳性组小鼠肝细胞中存在大量空泡样变性,细胞水肿且细胞质呈现疏松状态,而TCE+Tempol致敏阳性组表现为部分区域可见肝细胞水肿;溶剂组、致敏阴性组与空白组相比,小鼠肝组织中MDA活性、IL-22和IL-2含量均无统计学意义,致敏阳性组小鼠肝组织中MDA、IL-22和IL-2的相对表达量皆高于溶剂组和致敏阴性组(P<0.05),且TCE致敏阳性组高于同时点的TCE+Tempol致敏阳性组(P<0.05)。结论Tempol可能通过降低IL-22和IL-2水平对TCE引起的免疫性肝损伤起到保护作用。

Objective To explore the effects of intraperitoneal injection of tetramethylpiperidine(Tempol)on the interleukin(IL)-22 and IL-2 in the liver injury of trichloroethylene(TCE)sensitized mice and the influence of malondialdehyde(MDA)levels.Methods Forty 6~8 weeks Balb/c female mice were randomly sampled and divided into blank group,solvent group,TCE group and TCE+Tempol group to establish a TCE percutaneous sensitization model.The animals in TCE+Tempol group were injected intraperitoneally with Tempol solution(100 mg/kg)before the last challenge.24 hours after the last challenge,the animals were divided into sensitization positive group or sensitization negative group according to the skin sensitization result score.The pathology of liver sections was observed by HE staining method,serum was separated for the detection of aspartate aminotransferase(AST)and alanine aminotransferase(ALT),and MDA content in liver was detected by TBA method,IHC and Western blot method to detect the expression level of IL-22 and IL-2 in liver tissue.Results The sensitization rates of TCE group and TCE+Tempol group were 0.40 and 0.33,respectively.Pathological examination found that the liver cells of the blank group,solvent group and non-sensitized group had no obvious pathological changes.There was a large number of vacuolar degeneration in the liver cells of the TCE sensitization positive group,with cell edema and cytoplasm in a loose state,while the TCE+Tempol sensitization positive group showed hepatocyte edema in some areas;Compared with the blank group,the MDA activity,IL-22 and IL-2 content in the liver tissue of mice in solvent group and sensitization negative group were not statistically significant.The relative expression levels of MDA,IL-22 and IL-2 in the liver tissue of mice in the sensitization positive group were higher than those in the solvent group and the sensitization negative group(P<0.05),and the TCE sensitization positive group was higher than the TCE at the same point TCE+Tempol sensitization positive group(P<0.05).Conclusion Tempol may reduce the levels of IL-22 and IL-2 to protect the immune liver injury caused by TCE.