30例湿热蕴结型结肠癌患者的肠道菌群结构变化研究

Study on the Structural Changes of Intestinal Flora in 30 Colon Cancer Patients with Moisture and Heat Accumulation Syndrome

目的探讨湿热蕴结型结肠癌患者与健康人群的肠道菌群的差异。方法30例湿热蕴结型结肠癌患者的粪便样本做为观察组,收集13名健康人体检粪便样本为对照组,分别提取两组样本中细菌总DNA,采用16S rDNA测序技术对各组的粪便样本进行分析。结果与对照组比较,观察组肠道菌群中拟杆菌门、放线菌门、变形杆菌门、拟杆菌属、大肠杆菌/志贺氏菌属丰度明显增加(P<0.01)。Ⅱ期患者的双歧杆菌属、柯林斯菌属明显高于Ⅲ期患者(P<0.05)。结论湿热蕴结型结肠癌与肠道菌群结构紊乱相关,关联度较高的为条件致病菌大肠杆菌相对丰度的升高,益生菌双歧杆菌相对丰度的减少。

Objective To observe the difference of intestinal flora between colon cancer patients with moisture and heat accumulation syndrome(MHAS)and the healthy population.Methods The fecal samples of 30 colon cancer patients with MHAS were selected as an observation group,and 13 samples from the healthy subjects were selected as the control group.The total bacterial DNA was extracted from the two groups and analyzed by 16 S rDNA sequencing.Results Compared with the control group,the abundance of Bacteroidetes,Actinomycetes,Proteobacteria,Bacteroides,Escherichial Shigella in the intestinal flora of the observation group increased significantly(P<0.01).The contents of Bifidobacterium and Collinsella were significantly higher in stageⅡpatients than in stageⅢpatients(P<0.05).Conclusions Colon cancer patients with MHAS were associated with structural disorder of intestinal flora.Higher relativities existed in increased relative abundance of Escherichiacoli and decreased relative abundance of Bifidobacterium.