TTLL12过表达对口腔鳞癌SCC-25细胞株生物学特性的影响

Effects of overexpression of TTLL12 on the biological characteristics of oral squamous cell carcinoma SCC-25 cell line

目的检测微管蛋白酪氨酸连接酶类似物12(TTLL12)过表达对人舌磷癌SCC-25细胞株生物学特性的影响。方法建立TTLL12稳定过表达的SCC-25细胞株,采用间接免疫荧光法和Western blot检测TTLL12在SCC-25细胞内的分布,MTT检测沉默TTLL12对SCC-25细胞生长的影响,Boyden小室侵袭试验检测沉默TTLL12对SCC-25细胞侵袭的影响,采用SPSS10.0软件包对数据进行统计学分析。结果间接免疫荧光法和Western blot结果均显示TTLL12在细胞质、细胞核及细胞核膜均有分布。间接免疫荧光法结果显示α微管蛋白(α-tubulin)的分布与TTLL12重合。MTT实验结果显示沉默组(沉默TTLL12基因的SCC-25细胞)的SCC-25细胞生长较对照组(未作任何处理的SCC-25细胞)受到明显抑制(P<0.05)。Boyden小室侵袭实验结果显示沉默组的穿膜细胞百分比明显小于对照组(P<0.05)。结论沉默TTLL12可有效抑制SCC-25细胞的生长和侵袭能力。

Objective To detect the effect of overexpression of tubulin tyrosine ligase like protein 12(TTLL12)on the biological characteristics of SCC-25 cell line.Methods The SCC-25 cell line with stable overexpression of TTLL12 was established.Indirect immunofluorescence and Western blot were used to detect the distribution of TTLL12 in SCC-25 cells.MTT assay was used to detect the effect of silencing TTLL12 on the growth of SCC-25 cells.The Boyden chamber invasion test was used to detect the effect of silencing TTLL12 on the invasion of SCC-25 cells.The data was analyzed by using SPSS10.0 software package.Results The results of indirect immunofluorescence and Western blot showed that TTLL12 was distributed in the cytoplasm,nucleus and nuclear membrane.The results of indirect immunofluorescence showed that the distribution ofα-tubulin overlapped with TTLL12.The results of the MTT assay showed that the growth of SCC-25 cells in the silence group(SCC-25 cells that silenced the expression of TTLL12 gene)was significantly inhibited when compared with the control group(SCC-25 cells without any treatment),P<0.05.The results of Boyden chamber invasion test showed that the percentage of transmembrane cells in the silence group was significantly lower than that in the control group(P<0.05).Conclusion Silencing TTLL12 can effectively inhibit the growth and invasion of SCC-25 cells.