探索白细胞介素-1受体拮抗剂对大鼠角膜移植术后Treg的影响

Exploring the effect of interleukin-1 receptor antagonist on Treg after corneal transplantation in rats

目的探讨大鼠角膜移植术术后玻璃体腔内注射白细胞介素-1受体拮抗剂(IL-1ra)对白细胞介素-1β(IL-1β)、调节性T淋巴细胞(Treg)及排斥反应的影响。方法取心脏死亡供体SD大鼠角膜分别在24只受体SD大鼠内行穿透性角膜移植术,将受体大鼠分为磷酸缓冲盐溶液(PBS)组与IL-1ra组,术毕分别于玻璃体腔注射PBS或IL-1ra 1IU。术后1、3、5周,观察并记录角膜排斥反应指数(RI),取全血、淋巴结检测IL-1β水平与Treg数量。结果术后1、3、5周,两组全血、淋巴结IL-1β水平逐渐降低,Treg数量均逐渐升高,组内不同时间点两两比较差异有统计学意义(P<0.05)。术后1、3、5周,IL-1ra组全血、淋巴结中IL-1β水平较PBS组明显下降(P<0.05);IL-1ra组全血、淋巴结中Treg数量较PBS组升高,其中术后3、5周全血Treg数量两组间差异有统计学意义(P<0.05),术后1、3、5周淋巴结中Treg数量两组间差异有统计学意义(P<0.05)。术后3、5周,IL-1ra组RI明显低于PBS组(P<0.05)。结论大鼠角膜移植术术后玻璃体腔注射IL-1ra可减轻炎性反应,升高全血、淋巴结中Treg数量,减少排斥反应。

Objective To investigate the effects of vitreous cavity injection of interleukin-1 receptor antagonist(IL-1 ra)on interleukin-1β(IL-1β),regulatory T lymphocytes(Treg)and transplant rejection after corneal transplantation.Methods The cornea of cardiac death donor SD rat were transplanted on 24 recipient SD rats,the receptor rats were divided into the phosphate buffer saline(PBS)group and the IL-1 ra group,1 IU of PBS injection or IL-1 ra were injected into vitreous cavity after surgery.1,3,and 5 weeks after surgery,the corneal rejection index(RI)was observed and recorded,and the whole blood and lymph nodes were collected to detect the level of IL-1βand the number of Treg.Results The IL-1βlevel in the whole blood and lymph nodes of the two groups were grudually decreased at the time of 1,3 and 5 weeks after surgery,and the number of Treg were grudually increased,the differences in pairwise comparisons at different time point were statstically significant(P<0.05).1,3,and 5 weeks after operation,IL-1βlevels in the whole blood and the lymph nodes in the IL-1 ra group were significantly lower than those in the PBS group(P<0.05).The number of Treg in the whole blood and the lymph nodes in the IL-1 ra group were higher than that in the PBS group.Among them,the number of Treg in whole blood was significantly different between the two groups at3 and 5 weeks after surgery(P<0.05),and the number of Tregs in lymph nodes was significantly different between the two groups at 1,3,and 5 weeks after surgery(P<0.05).The RI of the IL-1 ra group was significantly lower than that of the PBS group at 3,5 weeks after surgery(P<0.05).Conclusion Intravitreal injection of IL-1 ra after corneal transplantation in rats can reduce inflammatory response,increase the number of Treg in whole blood,lymph nodes,and reduce rejection.