CIH对仔鼠胰腺基因芯片测序及相关生物信息学的影响研究

Study on the effect of CIH on the pancreatic gene chip sequencing and related bioinformatics of offspring rats

目的利用基因芯片测序技术筛选宫内慢性间断性缺氧(CIH)雄性仔鼠胰腺的差异表达基因(DEGs),分析相关生物信息。方法12只怀孕SD大鼠分为正常组和缺氧组,缺氧组制作CIH模型,提取两组分娩后第12周的雄性仔鼠胰腺组织总RNA,纯度验证合格后,进行基因芯片测序:包括基因芯片DEGs筛选、GO功能分类、pathway富集分析等,并验证结果的可靠性。结果缺氧组雄性仔鼠空腹血清胰岛素、空腹血糖、以稳态模式评估胰岛素抵抗(HOMA-IR)均高于正常组雄性仔鼠(P<0.05),差异有统计学意义。CIH后缺氧组雄性仔鼠胰腺较正常组雄性仔鼠胰腺的分子功能、细胞组分、生物学过程的分析均有改变。共检测23088个基因,表达明显上调的基因2628个、明显下调的2321个(Fold Change≥2且P≤0.05)。结论妊娠期CIH可致成年雄性仔鼠胰腺表达基因、GO功能分类、胰岛素分泌等pathway富集转录通路等的失调,成年雄性仔鼠呈现胰岛素抵抗。

Objective To screen the differentially expressed genes(DEGs)in the pancreas of male offspring with chronic intermittent hypoxia(CIH)in utero by using gene chip sequencing technology and analyze the relevant biological information.Methods Twelve pregnant SD rats were divided into the normal group and the hypoxic group.CIH model was made in the hypoxia group.The pancreas of male offspring were extracted at 12 weeks after delivery.After the purity of total RNA was verified,the gene chip sequencing was performed,including gene chip DEGs screening,GO(gene ontology)functional classification,pathway enrichment analysis,etc.,and the reliability of the results was verified.Results The fasting serum insulin,fasting blood glucose,and HOMA-IR of male offspring in the hypoxia group were higher than those in the normal group(P<0.05).After CIH,the molecular function,cell composition and biological process of the pancreas of the male offspring in the hypoxia group were changed when compared with that in the normal groups.There were 23088 gene were cletected,with 2628 up-regulated and 2321 down-regulated(Fold Change≥2,P≤0.05).Conclusion CIH during pregnancy can cause disorders of pathway-enrichment transcription pathways such as pancreatic gene expression,GO function classification,and insulin secretion in adult male offspring,led to insulin resistance in adult male offspring。