采前喷施LAE提高蓝莓冷藏品质及其通径分析

Path Analysis of Improving Cold Storage Quality of Blueberry by Preharvest Spraying LAE

目的探究Nα-月桂酰-L-精氨酸乙酯盐酸盐(Nα-lauroyl-L-arginine ethyl ester hydrochloride,LAE)处理蓝莓影响冷藏好果率及其作用机制。方法采用逐步多元回归和通径分析方法,研究采前喷施LAE(0,30,60,90 mg/L)对蓝莓冷藏期间果实品质理化指标的影响。结果LAE处理的蓝莓在冷藏过程中还原糖的含量高峰比对照组推迟10 d以上,有效地使贮藏前期果实保持了较高的硬度和POD活性。在整个冷藏期中,30~60 mg/L LAE处理蓝莓果实的·OH清除率与好果率之间呈极显著正相关关系(P<0.01)。30 mg/L LAE处理的蓝莓冷藏15 d后,果实的硬度、可溶性固形物和还原糖含量分别比对照组高45.27%,23.81%和28.57%,总酸含量下降37.50%,好果率(86.8%)比对照组高16.04%。结论采前喷施30 mg/L LAE能提高冷藏蓝莓的好果率,果实的·OH清除率是影响好果率的决策因子,果实硬度通过·OH清除率对蓝莓好果率产生了较大的间接正向作用。

In order to explore the effect of Nα-lauroyl-l-arginine ethyl ester hydrochloride(LAE)treatment on the good fruit rate of cold storage blueberry and its mechanism of action,the effects of preharvest spraying LAE(0,30,60 and 90 mg/L)on the physicochemical quality indexes of blueberry fruits during cold storage were studied by stepwise multiple regression and path analysis.During the cold storage,the peaks of reducing sugar content of three concentration levels of LAE treated blueberries were delayed more than 10 days than that of the control,which effectively maintained the high firmness,·OH clearance rate and POD activity of blueberry fruits in the early stage of storage.During the whole cold storage period,there was a significant positive correlation between·OH clearance rate and good fruit rate of 30-60 mg/L LAE treated blueberry fruits(P<0.01).After 15 days of cold storage,the hardness,soluble solids and reducing sugar content of blueberry treated with 30 mg/L LAE were 45.27%,23.81%and 28.57%higher than those of the control group,the total acid content decreased by 37.50%,and the good fruit rate was 16.04%higher than that of the control group.Preharvest spraying 30 mg/L LAE could improve the good fruit rate of cold storage blueberry,·OH clearance rate of the fruit was the decision factor that affected the good fruit rate,and the fruit hardness through·OH clearance rate also had an indirect positive effect on the good fruit rate of blueberry.