从健康人尿液筛选年龄相关蛋白质

Screening aging related proteins in urine from healthy adults

目的筛选健康成人尿液中与年龄相关的差异表达蛋白质,探讨成人因年龄增长而发生的蛋白质改变。方法选取14名健康男性及15名健康女性,按年龄各分为3组(20~30、40~50和≥60周岁),用非标记定量蛋白质组学技术进行尿液蛋白质组分析,筛选年龄相关尿液蛋白。结果从男性尿液中共获得100个与年龄增长相关的尿蛋白,其中上调66个,下调34个。从女性尿液中共获得94个与年龄增长相关尿蛋白,其中上调79个,下调15个。获得男女共有的年龄增长相关尿蛋白12个。蛋白功能分析显示,这些蛋白与凝血系统、蛋白泛素化、LXR/RXR激活、糖酵解等通路相关。结论尿液中存在与年龄增长相关的蛋白质,有可能成为衰老相关的尿液标志物,并为后续疾病生物标志物发现提供基础。

Objective To screen the differential proteins related to aging in human urine and to find out the functional changes of proteins related to aging.Methods Fourteen healthy men and 15 healthy women were enrolled and divided them into three groups according to their ages(20-30,40-50 and over 60 years old).The differential proteins were screened by label-free quantification and 2DLC-MS/MS.Results In male group,there were 100 proteins differentially expressed in three groups,66 were over-expressed and 34 were under-expressed.In female group,there were 94 proteins differentially expressed in three groups,79 were overexpressed and 15 were under-expressed.There were 12 differential expressed proteins related to aging in both male and female groups.These protein pathways mainly focused on coagulation system,protein ubiquitination pathway,LXR/RXR activation and lactose degradation III,etc.Conclusions Proteins related to aging can be found in urine,which may be used as biomarkers of aging and providing basis for subsequent discovery of disease biomarkers.