piRNAs在阿尔茨海默病患者脑中的差异表达

Differential expression of piRNAs in brain from patients with Alzheimer's disease

目的探讨piRNAs在阿尔茨海默病(AD)患者与对照人群脑组织中的表达差异,并研究这些piRNAs与AD的相关性。方法选取年龄、性别比例匹配的6个AD样本(病理AD)与7个对照样本,取相同位置的前额叶脑组织提取RNA,加尾制备成piRNA后反转录成cDNA。基于前期研究结果选取10个在人脑中表达的piRNA:DQ571030、DQ597973、DQ576872、DQ597479、DQ586404、DQ590835、DQ600318、DQ581610、DQ586113和DQ571669,利用real-time PCR检测这些piRNA是否存在表达量的差异,对差异显著的piRNA继续进行相关的mRNA检测。结果选择的10个piRNA在AD组均出现表达量降低的趋势,其中4个piRNA DQ597973、DQ576872、DQ597479和DQ600318在AD组较对照组降低(P<0.05)。进一步对表达量相差最显著的DQ600318相关的mRNA LRRC37A3进行检测,LRRC37A3在AD组的表达量显著低于对照组(P<0.01)。结论DQ600318和LRRC37A3出现的表达量降低可能和AD相关,对DQ600318的表达检测可能成为潜在的AD疾病标志物。

Objective To investigate the differential expression of piRNAs in brain tissues from patients with Alzheimer's disease(AD),and to study the roles of these piRNAs related to AD.Methods With age and sex matching,6 AD(pathological AD)and 7 control subjects were selected.RNAs were converted into piRNAs after extracting from the prefrontal lobe brain tissues,then piRNAs were reversed transcription into cDNA.Based on preliminary results,10 piRNAs expressed in brain tissues were selected:DQ571030,DQ597973,DQ576872,DQ597479,DQ586404,DQ590835,DQ600318,DQ581610,DQ586113 and DQ571669.The expression of piRNAs was detected by real-time PCR.Those mRNA related with piRNAs of significant differences were further studied.Results A trend of decrease was found in the expression of all the 10 piRNAs in the AD group.The expression of 4 piRNAs DQ597973,DQ576872,DQ597479 and DQ600318 were significantly decreased in the AD group(P<0.05).The related mRNA LRRC37A3 of DQ600318 was further studied.DQ600318 showed the most significant difference inexpression.The expression of LRRC37A3 was also significantly decreased in the AD group(P<0.01).ConclusionsThe decreasing expression of DQ600318 and LRRC37A3 is potentially related to AD,and the detection of DQ600318 may function as a marker of AD pathogenesis.