流式细胞术检测CD161对结核病的诊断效能

Efficacy of CD161 by flow cytometry in the diagnosis of active tuberculosis

目的探讨流式细胞术检测CD161对活动性肺结核的诊断效能,并比较其与其他检测方法的差异。方法采用流式细胞术检测138例体检健康者(健康对照组)和178例结核病患者(结核病组)全血细胞中CD161的表达,同时比较CD161检测技术、GeneXpert、结核分枝杆菌(TB)-DNA、痰涂片和γ-干扰素释放试验(IGRAs)对结核病的检测阳性率,以及在菌阴性肺结核患者和菌阳结核患者中的阳性率。结果CD161诊断活动性肺结核患者的灵敏度为72.47%,特异度为71.01%;CD161检测技术、GeneXpert、TB-DNA、痰涂片和IGRAs方法在检测结核患者中的阳性率分别为72.47%、50.34%、40.62%、33.33%、67.46%;在菌阳性肺结核患者中,GeneXpert检测阳性率最高,为97.96%,在菌阴性肺结核患者中,CD161检测技术阳性率最高,为72.64%。结论流式细胞术检测CD161对活动性肺结核有很高的诊断价值,可用于健康人群的体检筛查。

Objective To investigate the efficacy of CD161 by flow cytometry in the diagnosis of active tuberculosis,and to compare the difference between CD161 and other detection methods.Methods Flow cytometry was used to detect the expression of CD161 in peripheral blood of 138 healthy people(healthy control group)and 178 tuberculosis patients(tuberculosis group).The positive rates of CD161 test,GeneXpert,TB-DNA,sputum smear andγ-interferon release test(IGRAs)were compared for tuberculosis,as well as positive rates in negative and positive tuberculosis patients.Results The sensitivity and specificity of CD161 for active pulmonary tuberculosis were 72.47%and 71.01%,respectively.The positive rates of CD161,GeneXpert,TB-DNA,sputum smear and IGRAs were 72.47%,50.34%,40.62%,33.33%and 67.46%respectively.The positive rate of GeneXpert(97.96%)was the highest in bacteria positive patients and CD161(72.64%)was the highest in bacteria negative patients.Conclusion Flow cytometry detection of CD161 has a good diagnostic value for active tuberculosis,which could be used in physical examination of healthy people.