土贝母含药血清对人肺癌细胞增殖和凋亡的影响

Effects of Tubeimu medicated serum on proliferation and apoptosis of human lung cancer cells

目的观察土贝母含药血清对人肺癌细胞增殖、凋亡的影响,初步探讨其作用机制。方法采用灌胃SD大鼠土贝母水提物的方法制备其含药血清。应用不同浓度(0.0%、2.5%、5.0%、10.0%、20.0%、40.0%)含药血清处理人肺癌A549和H1299细胞。采用MTT、免疫荧光法观察含药血清对人肺癌细胞增殖的影响,采用流式细胞术检测含药血清对人肺癌细胞凋亡的影响,采用Western blot方法检测含药血清对肺癌细胞中凋亡相关的裂解型胱天蛋白酶(Cleaved-Caspase-3、Cleaved-Caspase-8、Cleaved-Caspase-9、Bax和Bcl-2)蛋白表达水平的影响。结果土贝母含药血清可明显抑制人肺癌A549和H1299细胞的增殖,诱导其凋亡;土贝母含药血清处理人肺癌细胞的Cleaved-Caspase-3、Cleaved-Caspase-9和Bax蛋白水平增加,Bcl-2蛋白水平降低。结论土贝母含药血清可明显抑制人肺癌A549和H1299细胞的增殖,诱导其凋亡,其机制可能与细胞线粒体途径诱导细胞凋亡有关。

Objective To observe the effects of Tubeimu medicated serum on the proliferation and apoptosis of human lung cancer cells,and to explore its mechanism.Methods The Tubeimu medicated serum was prepared by intragastric administration of water extract of Tubeimu in SD rats.Human lung cancer cells A549 and H1299 were treated with different concentrations of Tubeimu medicated serum(0.0%,2.5%,5.0%,10.0%,20.0%,40.0%).MTT and immunofluorescence were used to observe the effect of drug serum on the proliferation of human lung cancer cells.Flow cytometry was used to detect the effect of Tubeimu medicated serum on apoptosis of human lung cancer cells.Western blot was used to detect the effect of Tubeimu medicated serum on the protein expression levels of apoptotic Cleaved-Caspase-3,Cleaved-Caspase-8,Cleaved-Caspase-9,Bax and Bcl-2 in lung cancer cells.Results The Tubeimu medicated serum could significantly inhibit the proliferation and induce the apoptosis of human lung cancer cells A549 and H1299.The Cleaved-Caspase-3,Cleaved-Caspase-9 and Bax levels of human lung cancer cells A549 and H1299 showed increased while Bcl-2 levels showed decreased after treating with Tubeimu medicated serum.Conclusion The Tubeimu medicated serum can significantly inhibit the proliferation and induce the apoptosis of in human lung cancer cells A549 and H1299.