Duffy、Diego等4种血型系统基因频率及多态性研究

Gene frequency and polymorphism of four blood group systems,such as Duffy and Diego

目的了解岳阳汉族人群Kidd(Jk^(a)/Jk^(b))、Duffy(Fy^(a)/Fy^(b))、Diego(Di^(a)/Di^(b))、Dombrock(Do^(a)/Do^(b))4种红细胞血型系统的基因频率和多态性特点,为建立该地区稀有血型库提供数据支持。方法采用聚合酶链式反应-序列特异性引物法(PCR-SSP法)对303名岳阳汉族RhD(+)固定献血者进行4种血型系统基因分型。结果岳阳汉族人群Duffy血型的基因频率为Fy^(a)=0.9472,Fy^(b)=0.0528;Dombrock血型基因频率为Do^(a)=0.0842,Do^(b)=0.9158;Diego血型基因频率为Di^(a)=0.0247,Di^(b)=0.9752;Kidd血型基因频率为Jk^(a)=0.4802,Jk^(b)=0.5198。结论岳阳汉族人群Duffy、Diego、Kidd和Dombrock血型系统基因呈多态性分布。

Objective To investigate gene frequency^(a)nd gene polymorphism of the Kidd(Jk^(a)/Jk^(b)),Duffy(Fy^(a)/Fy^(b)),Diego(Di a/Di^(b))and Dombrock(Do^(a)/Do^(b))blood group systems in Yueyang Han population.The data of this study can be used to initialize the establishment of regional rare blood donor programme.Methods The genotypes of ten blood groups of 303 RhD(+)samples from Yueyang regular blood donors were analyzed by PCR-SSP.Results The gene frequencies of ten blood group systems were as follows.Duffy:Fy^(a)=0.9472,Fy^(b)=0.0528;Dombrock:Do^(a)=0.0842,Do^(b)=0.9158;Diego:Di a=0.0274,Di^(b)=0.9752;Kidd:Jk^(a)=0.4802,Jk^(b)=0.5198.Conclusion The population distribution of Duffy,Diego,Kidd and Dombrock^(b)lood group systems in Yueyang Han population are polymorphic.