正己烷接触对血清神经元特异性烯醇化酶和神经丝蛋白的影响

Serum neuron-specific enolase and neurofilament level in workers exposed to n-hexane

目的探讨正己烷接触对人体血清神经元特异性烯醇化酶(neuron-specific enolase,NSE)及神经丝蛋白(neurofilament,NF)的影响。方法应用酶联免疫吸附法分别测定接触正己烷且出现临床症状及异常体征的83名工人及不接触职业病危害因素的48名对照组员工的NSE和NF,并分析工龄和以上两个指标的关系。结果接触组工作场所空气中正己烷时间加权平均浓度为0.54~70.00 mg/m3,平均(25.1±9.8)mg/m3,均未超过职业接触限值。接触组所在的生产车间共有工人577人,出现一项或以上临床症状及异常体征的有83例(占14.3%)。接触组血清NSE、NF质量浓度均明显高于对照组,差异有统计学意义(P<0.05)。随着工龄的增长,接触组工人血清NSE、NF有增高的趋势(P<0.01)。结论接触正己烷可能导致工人外周血NSE、NF表达水平升高,两者可作为长期接触正己烷导致外周神经病变的早期效应标志物。

Objective To study the effect of n-hexane exposure on serum level of neuron specific enolase(NSE) and neurofilament(NF). Methods A total of 83 n-hexane exposed workers who had clinical symptoms and 48 non-exposed workers from a factory in Shanghai were studied. Serum NSE and serum NF were determined by ELISA. The relationship between exposure time and the two indicators was analyzed. Results The airborne time weighted average concentration of n-hexane at workplaces varied from 0.54 to 70.00 mg/m3,with an average of(25.1 ± 9.8)mg/m3,which did not exceed the occupational exposure limit. Among 577 exposed workers,83 workers(the study subjects,accounting for 14.3 %) had one or more clinical symptoms and abnormal signs. The serum NSE and NF concentrations of workers in exposed group were significantly higher than those in the control group(P < 0.05). The serum NSE and NF level in the exposed group had an increasing tendency with the increment of exposure time(P < 0.01). Conclusions Exposure to n-hexane may increase the expression level of NSE and NF in peripheral blood among occupationally exposed workers,which could be used as early effect markers of peripheral neuropathy caused by long-term exposure to n-hexane.