丝瓜花不同溶剂提取物抗氧化活性及其酚类成分分析

Evaluation of Antioxidant Activities of Various Solvent Extract from Luffa cylindrica(L.)Roem and Its Phenolic Components Analysis

以无水乙醇、70%乙醇、40%乙醇和水分别对丝瓜花进行提取,并测定各提取物中总酚、总黄酮含量,采用DPPH·清除能力、超氧化物自由基清除能力、FRAP抗氧化能力和亚铁离子螯合能力等方法评价其抗氧化活性,通过HPLC分析其中酚类成分。结果表明,70%和40%乙醇提取物总酚及总黄酮含量较高,而且DPPH·清除活性、FRAP还原力最强,40%乙醇提取物、水提物分别具有最强的超氧阴离子自由基清除活性和亚铁离子螯合能力。总酚含量与DPPH·清除活性之间存在较强的相关性(R^(2)=0.8688)。HPLC分析检出提取物中9种酚类化合物,其中含量最高的为杨梅素。试验结果说明,70%乙醇、40%乙醇可能是丝瓜花抗氧化性物质提取的较好溶剂。结果表明丝瓜花是一种较好的抗氧剂资源。

The antioxidant activities of ethanol extract,70%ethanol extract,40%ethanol extract and water extract from Luffa cylindrica(L.)Roem were investigated for the first time.Total phenolic content(TPC),total flavonoid content(TFC),DPPH radical scavenging activities,superoxide radical scavenging activities,ferric reducing-antioxidant power(FRAP)assay and ferrous ions chelating activities were determined for all the extracts.The results showed that 70%ethanol extract and 40%ethanol extract had the highest TPC and TFC,and exhibited the best DPPH radical scavenging activities and superoxide radical scavenging activities.The 40%ethanol extract also showed the best FRAP activity,while the water extract exhibited highest metal chelating activity antioxidant abilities.Correlation analysis suggested that TPC was highly correlated with DPPH radical scavenging activity(R^(2)=0.8688).In addition,9 phenolic compounds were identified and quantified by HPLC from these extracts,and the myricetin was present at the highest concentration in all extracts.It seemed that 40%ethanol and 70%ethanol were more suitable for extraction compounds with radical scavenging properties from Luffa cylindrica(L.)Roem flower.The results indicated that the extracts of Luffa cylindrica(L.)Roem might be a useful potential source of natural antioxidant ingredients.