肥胖合并动脉粥样硬化大鼠血管旁脂肪组织中趋化因子chemerin的表达变化

Changes of Chemokine Chemerin Expression in Perivascular Adipose Tissue of Obese Rats with Atherosclerosis

目的:通过构建肥胖合并动脉粥样硬化大鼠模型,评估模型血管旁脂肪组织中趋化因子chemerin基因及蛋白的表达变化。方法:建立肥胖合并动脉粥样硬化大鼠模型;于模型构建不同时期(8周、12周、16周及24周)取胸主动脉旁脂肪组织,应用realtime-PCR检测chemerin的m RNA表达变化;应用免疫组织化学染色的方法检测蛋白表达变化。结果:与对照组相比,造模8周时模型组大鼠血管旁脂肪组织中chemerin的m RNA表达较对照组明显增加(P=0.031),造模12周及16周时无明显差异,造模24周时表达明显增加(P<0.001);Pearson直线相关分析显示,血管旁脂肪组织中chemerin的m RNA表达与其蛋白表达、血管旁脂肪质量、内脏脂肪质量、皮下脂肪质量、低密度脂蛋白胆固醇浓度及主动脉中层厚度呈正相关;与血管旁脂肪组织中脂联素的m RNA表达及高密度脂蛋白浓度呈负相关。结论:成功建立了肥胖合并动脉粥样硬化大鼠模型;伴随造模时间的延长,模型大鼠血管旁脂肪组织明显增多,动脉粥样硬化逐渐加重,血管旁脂肪组织中chemerin的表达升高。血管旁脂肪组织中chemerin可能参与了动脉粥样硬化发生发展过程。

Objective: To evaluate the expression of chemokine chimerin gene and protein in perivascular adipose tissue of obese rats with atherosclerosis. Methods: The rat model of obesity and atherosclerosis was established. The paraaortic adipose tissue was taken at different stages(8 weeks, 12 weeks, 16 weeks and 24 weeks), and the expression of chimerin m RNA was detected by real-time PCR and the expression of protein was detected by immunohistochemistry. Results: Compared with the control group, the expression of chimerin m RNA in the perivascular adipose tissue of the model group was significantly increased(P=0.031) at 8 weeks after the establishment of the model, and there was no significant difference at 12 and 16 weeks after the establishment of the model, but it was significantly increased at 24 weeks after the establishment of the model(P<0.001). Pearson linear correlation analysis showed that the expression of chimerin m RNA in perivascular adipose tissue was positively correlated with its protein expression, perivascular adipose mass,visceral adipose mass, subcutaneous adipose mass, low-density lipoprotein cholesterol concentration and aortic middle thickness, and negatively correlated with adiponectin m RNA expression and high-density lipoprotein concentration. Conclusion: The rat model of obesity with atherosclerosis was established successfully. With the prolongation of the time of establishing the model, the perivascular adipose tissue increased significantly, the atherosclerosis increased gradually, and the expression of chemerin in the perivascular adipose tissue increased. Chemerin in perivascular adipose tissue may be involved in the development of atherosclerosis.