癌组织中KLF9、TFAP4基因表达在非小细胞肺癌患者预后中的应用价值

The prognostic significance of KLF9 and TFAP4 gene expression in cancer tissues for patients with non-small cell lung cancer

目的探究癌组织中Kruppel样转录因子9(KLF9)、转录因子激活增强子结合蛋白4(TFAP4)基因表达在非小细胞肺癌(NSCLC)患者预后中的应用价值。方法选取NSCLC患者94例作为研究对象,检测癌组织及癌旁组织中KLF9、TFAP4 mRNA表达量。并以基因表达量均数为界分为低表达组、高表达组。比较两组年龄、性别、病理类型、TNM分期、淋巴结转移、分化程度、肿瘤最大径、癌组织中KLF9、TFAP4 mRNA表达量,采用Pearson分析癌组织中KLF9、TFAP4 mRNA与TNM分期、淋巴结转移、分化程度、肿瘤最大径的相关性,采用logistic多元回归方程行多因素分析,采用Kaplan-Meier曲线进行生存分析。结果癌组织KLF9 mRNA表达水平低于癌旁组织,TFAP4 mRNA表达水平高于癌旁组织(P<0.05);KLF9、TFAP4 mRNA高表达组TNM分期、淋巴结转移、分化程度、肿瘤最大径与低表达组相比,差异有统计学意义(P<0.05);KLF9 mRNA与TNM分期、淋巴结转移、肿瘤最大径呈负相关,与分化程度呈正相关,TFAP4 mRNA与TNM分期、淋巴结转移、肿瘤最大径呈正相关,与分化程度呈负相关(P<0.05);KLF9 mRNA、TFAP4 mRNA高表达与低表达患者生存曲线比较,差异有统计学意义(P<0.05)。结论NSCLC患者癌组织中KLF9、TFAP4基因表达与TNM分期、淋巴结转移、肿瘤最大径、分化程度显著相关,可影响患者预后,靶向KLF9、TFAP4基因可能有助于预后的改善。

Objective To investigate the prognostic values of Kruppel-like transcription factor 9(KLF9)and transcription factor activation enhancer binding protein 4(TFAP4)gene for patients with non-small cell lung cancer(NSCLC).Methods A total of 94 patients with NSCLC were selected.The expressions of KLF9 and TFAP4 mRNA in cancer tissues and adjacent tissues were assessed.The patients were divided into the low expression group and the high expression group based on the mean of gene expression.The age,gender,pathological type,TNM stage(the seventh edition of the NSCLC TNM staging system standard proposed by the 2009 World Lung Cancer Congress),lymph node metastasis,degree of differentiation,tumor diameter,and KLF9 and TFAP4 mRNA expression in cancer tissues were compared between the two groups.The correlation of KLF9 and TFAP4 mRNA with TNM stage,lymph node metastasis,degree of differentiation,and tumor diameter was analyzed by Pearson analysis.Logistic multiple regression equations were used for multivariate analysis,and Kaplan-Meier curves were used for survival analysis.Results The expression level of KLF9 mRNA in cancer tissues was significantly lower than that in adjacent tissues,and the expression level of TFAP4 mRNA was significantly higher than that in adjacent tissues(P<0.05).There were significant differences in TNM stage,lymph node metastasis,degree of differentiation,and tumor diameter between high expression group and low expression group(P<0.05).KLF9 mRNA was significantly negatively correlated with TNM stage,lymph node metastasis,and tumor diameter;and significantly positively correlated with the degree of differentiation(P<0.05).TFAP4 mRNA was significantly positively correlated with TNM stage,lymph node metastasis,and tumor diameter;and significantly negatively correlated with the degree of differentiation(P<0.05).The survival curves of patients with high expression of KLF9 mRNA and TFAP4 mRNA was significantly differed with those with low expression(P<0.05).Conclusion The expression of KLF9 and TFAP4 genes in cancer tissues of NSCLC patients is significantly correlated with TNM stage,lymph node metastasis,tumor diameter,and degree of differentiation;which can affect the prognosis.Targeting therapy of KLF9 and TFAP4 genes may contribute to the improvement of prognosis.