甘油葡萄糖苷αGG抗氧化性能及其对UV致细胞损伤保护修复的研究

Antioxidantion property of 2-O-(α-D-glucopyranosyl)-sn-glycerol and repair of cell damage caused by UV radiation

笔者探讨了酶法制得的甘油葡萄糖苷(αGG)的抗氧化性能及其对紫外辐射致细胞损伤保护修复的促进作用。通过测定对DPPH自由基(DPPH·)、羟基自由基(·OH)和超氧阴离子自由基(·O_(2)~(-))的清除能力来研究αGG的抗氧化活性。结果表明:αGG对3种自由基均存在不同程度的清除能力,且抗氧化性与其质量浓度呈正相关;当αGG为20 mg/mL时,对3种自由基的清除率分别达到29.8%、80.0%和20.0%。同时,以L929细胞为实验材料,建立了紫外辐射对L929细胞的损伤模型,采用MTT等方法研究不同浓度的αGG对L929细胞紫外损伤的保护与修复作用;质量浓度达到20 mg/mL时,经过αGG预处理和后处理的细胞存活率分别提升了59.6%和54.5%。结果表明,特定浓度的αGG不但可以促进细胞增殖,还对紫外辐射致细胞损伤具有一定的保护修复作用。

The antioxidation of 2-O-(α-D-glucopyranosyl)-sn-glycerol(hereinafter referred to asαGG obtaining by bio-production)and the protection and repair of cell damage induced by UV radiation were also investigated.The scavenging properties ofαGG on DPPH free radical,hydroxyl free radical and superoxide anion free radical were tested.Results showed thatαGG has certain degree of scavenging activity on the above free radicals,and the scavenging rate was positively correlated with its mass concentration.WhileαGG was 20 mg/mL,the scavenging rates of the three free radicals were 29.8%,80.0%and 20.0%,respectively.On the other hand,this experiment used L929 cells as experimental materials to establish a model of L929 cells related to UV radiation.MTT assay was used to test the protection and repair abilities of different concentrations ofαGG.Results showed thatαGG not only promoted cell proliferation,but also showed effects on repair and protection of UV-induced cell damage.WhenαGG concentration reached 20 mg/mL,the viabilities of cells withαGG pretreated and post-treated were significantly increased by 59.6%and 54.5%,respectively.