怀山药内生真菌Alternaria sp.L12的次生代谢产物研究

Secondary metabolites of endophytic Alternaria sp.L12 from dioscoreae rhizoma

目的研究怀山药内生真菌Alternaria sp.L12的次生代谢产物。方法利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和半制备HPLC等色谱技术分离纯化Alternaria sp.L12的大米发酵产物,运用波谱分析技术鉴定化合物结构。结果从Alternaria sp.L12的大米发酵物的乙酸乙酯萃取部位中分离得到8个化合物,分别鉴定为tricycloalternarene 1b(1)、alternariol-9-O-monomethyl ether(2)、过氧麦角甾醇(3)、alternariol(4)、对羟基苯乙酸(5)、邻羟基苯乙酸(6)、苯乙酸(7)和对羟基苯乙醇(8)。结论以上8个化合物均系首次从怀山药内生链格孢属真菌的发酵物中分离得到。

Objective To determine the secondary metabolites from Alternaria sp.L12,an endophytic fungus from dioscoreae rhizoma.Methods The secondary metabolites of Alternaria sp.L12 were extracted and separated by silica gel column chromatography,Sephadex LH-20 column chromatography,and semi-preparative HPLC.The isolates were identified by spectra analysis.Results Eight compounds were isolated from the ethyl acetate extract of Alternaria sp.L12,and were identified as tricycloalternarene 1b(1),alternariol-9-O-monomethyl ether(2),ergosterol peroxide(3),alternariol(4),4-hydroxyphenylacetic acid(5),2-hydroxyphenylacetic acid(6),phenylacetic acid(7),and 4-hydroxyphenethyl alcohol(8).Conclusion All the compounds have been isolated from dioscoreae rhizoma for the first time.