染料木黄酮对乳腺癌细胞增殖、凋亡、迁移和侵袭的影响

Effect of genistein on the proliferation,apoptosis,migration and invasion of breast cancer cells

目的探讨染料木黄酮(GEN)对乳腺癌MCF-7和MDA-MB-231细胞增殖、凋亡、侵袭和迁移的影响及其相关机制。方法CCK-8、平板克隆实验、线粒体膜电位检测试剂盒检测细胞的增殖凋亡;划痕实验、Transwell实验检测细胞迁移侵袭;Western blot法检测凋亡和转移相关蛋白表达水平。结果GEN对两种细胞的增殖有明显抑制作用,且呈剂量依赖关系。随着GEN浓度增加,乳腺癌细胞的克隆形成能力逐步降低(P<0.05),凋亡数量增加,迁移侵袭能力逐渐减弱。GEN显著提高BAX、Caspase 3、Cleaved-caspase 3、E-Cadherin蛋白表达水平,降低Bcl2、Bcl-XL、N-Cadherin、Vimentin、Twist、Snail、Slug、MMP-2、MMP-9蛋白表达水平(P<0.05)。结论GEN可能通过影响细胞凋亡相关蛋白、EMT相关蛋白的表达抑制乳腺癌细胞MCF-7和MDA-MB-231的增殖与侵袭迁移能力,促进其凋亡,进而抑制肿瘤的发展。

Objective To determine the effect and mechanism of genistein(GEN)on the proliferation,apoptosis,invasion and migration of human breast cancer cell lines MCF-7 and MDA-MB-231.Methods The cell proliferation was assessed by CCK-8 assay and plate clone formation experiment.The apoptosis was evaluated by mitochondrial membrane potential detection kit.Cell scratch test and Transwell experiment were used to detect the migration and invasion of the cells.Western blot was conducted to detect the expression level of apoptosis-related proteins and metastasis-related proteins.Results GEN inhibited the proliferation of MCF-7 and MDA-MB-231 in a dose-dependent manner.Treatment of breast cancer cells with GEN resulted in the promotion of apoptosis as well as reduced cell migration,invasion and colony formation.GEN upregulated the protein expression of BAX,Caspase 3,Cleaved-caspase 3 and E-Cadherin,but downregulated the protein expression of Bcl2,Bcl-XL,N-Cadherin,Vimentin,Twist,Snail,Slug,MMP-2 and MMP-9(P<0.05).Conclusion GEN may inhibit the proliferation,invasion and migration of breast cancer cell lines MCF-7 and MDA-MB-231 by affecting the expression of apoptosis-related proteins,EMT-related proteins and promoting their apoptosis,and further inhibiting tumor development.