一株地衣内生真菌Daldinia childiae的化学成分

Chemical constituents of an endolichenic fungus Daldinia childiae(Ascomycota)

本研究对分离自星点梅衣属地衣Puncteliasp.的一株内生真菌蔡氏轮层炭壳菌Daldiniachildiae进行大米固体发酵培养,乙酸乙酯萃取发酵产物,采用硅胶柱色谱法、重结晶等方法分离纯化次生代谢产物,结合核磁共振波谱与质谱技术对单体化合物进行结构解析,运用DPPH法测定化合物的自由基清除能力。结果分离得到8个化合物,分别鉴定为4-hydroxy-benzeneethanol(1)、4-methoxy-5-carbomethoxy-6-pentyl-2H-pyran-2-one(2)、1-(2,6-dihydroxyphenyl)butan-1-one(3)、8-methoxy-1-naphthol(4)、1,8-dimethoxynaphthalene(5)、methyl 2-undecenoate-3-undecanoate-cyclohexyl-1,4-dienyl ester(6)、uracil(7)和aceticacid(8),化合物1、6和8均首次从轮层炭壳菌属Daldinia中分离得到;化合物4在浓度为3mg/mL时具有较强的自由基清除活性,化合物3和5具有轻微的自由基清除能力。本研究为进一步挖掘轮层炭壳菌属的代谢产物奠定基础。

The endolichenic fungus Daldinia childiae isolated from Punctelia sp.was cultivated by rice solid medium and the fermentative product was extracted with ethyl acetate.The secondary metabolites purified by the method of silica gel column chromatography and recrystallization were identified by combining nuclear magnetic resonance spectroscopy with mass spectrometry.The free radical scavenging ability of the compounds was determined by DPPH method.Eight compounds were isolated from Daldinia childiae,and they were determined as 4-hydroxybenzeneethanol(1),4-methoxy-5-car-bomethoxy-6-pentyl-2 H-pyran-2-one(2),1-(2,6-dihydroxyphenyl)butan-1-one(3),8-methoxy-1-naphthol(4),1,8-dimethoxynaphthalene(5),methyl 2-undecenoate-3-undecanoate-cyclohexyl-1,4-dienyl ester(6),uracil(7)and acetic acid(8).The compounds 1,6 and 8 were isolated from the genus of Daldinia for the first time.The compound 4 under concentration of 3 mg/mL showed siginificant activity of free radical scavenging.The compound 3 and 5 exhibited slight free radical scavenging ability.