响应面法优化地衣Umbilicaria muehlenbergii多糖提取工艺及其体外活性研究

Optimization of extraction process and in vitro activities of polysaccharides from Umbilicaria muehlenbergii(Ascomycota)

研究石耳属地衣Umbilicaria muehlenbergii多糖的提取工艺及其体外抗氧化、抗肿瘤能力。在单因素试验基础上,通过Box-Behnken design(BBD)和响应面分析法优化得出热水浸提法提取U.muehlenbergii多糖的最佳工艺条件。通过测定地衣多糖对1,1-二苯基-2-苦肼基自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH)、羟自由基、超氧阴离子和亚硝酸盐的清除率,对其体外抗氧化能力、抗肿瘤能力进行了评价。结果表明,U.muehlenbergii多糖提取最佳工艺参数为温度90℃,时间每次4.5h,液固比40:1,提取次数5次。在此工艺条件下,U.muehlenbergii多糖实际提取率为10.63%。同时,该多糖具有较好的抗氧化、抗肿瘤能力,是一种潜在的开发资源。

The extraction process and in vitro activities of polysaccharides from Umbilicaria muehlenbergii were investigated.The optimization of the extraction parameters was investigated by single-factor and response surface methodology.In vitro activities of the polysaccharides were evaluated by the methods of scavenging DPPH,hydroxyl radical,superoxide anion and nitrite.Results showed that the optimal parameters were obtained under the conditions of 5 extraction cycles each sustaining for 4.5 h at a liquid-solid ratio of 40:1 at 90°C.The polysaccharide yield extracted was 10.63%.The polysaccharides exhibited significant antioxidant and antitumor activities in vitro,and hopefully could be developed as a product with potential medicinal value.