拮抗临床致病菌的一株地衣内生真菌

An endolichenic fungus inhibiting clinical pathogenic bacteria

本研究运用组织分离法对采自贵州省都匀市斗篷山景区的地衣进行内生真菌分离,乙酸乙酯萃取菌株液体发酵物,通过打孔法筛选地衣内生真菌提取物并对9株临床致病菌株及耐药菌株进行抑菌活性实验。结果表明一株分离自星点梅衣属地衣Punctelia sp.的内生真菌DPS-165-9对其中的6株显示抑制活性。此6株菌为金黄色葡萄球菌Staphylococcus aureus 25923和6538、耐药表面葡萄球菌Staphylococcus epidermidis Z12、耐药布氏杆菌Brucella sp.B103、耐药枯草芽孢杆菌Bacillus subtilis 163和耐药藤黄微球菌Micrococcus luteus 261。经鉴定DPS-165-9为蔡氏轮层炭壳菌Daldinia childiae。本研究为下一步挖掘新的抗菌物质奠定基础。

An endolichenic fungus strain Daldinia childiae DPS-165-9 was isolated from lichen Punctelia sp.collected from the Cloak Mountain Scenic Area in Duyun,Guizhou.The liquid-fermented product was extracted by ethyl acetate.Inhibitory activity of the extract against pathogenic and drug-resistant bacterial strains were performed by punch method.The results showed that D.childiae DPS-165-9 could inhibit Staphylococcus aureus 25923 and 6538,drug-resistant Staphylococcus epidermidis Z12,drug-resistant Brucella sp.B103,drug-resistant Bacillus subtilis 163 and drug-resistant Micrococcus luteus 261.The fungus is probably developable as a new antimicrobial agent.