驯化对生物阴极微生物燃料电池中阴极微生物群落多样性的影响

The influence of domestication on the diversity of cathodic microbial community in biocathode microbial fuel cell

【背景】生物阴极微生物燃料电池因其构造成本低和阴极可持续性发展的优点而成为一种很有前途的废水处理系统,但阴极微生物的氧化还原性能限制了其在实际应用中的推广。【目的】为了提高生物阴极的性能,需要深入了解影响阴极氧化还原性能的微生物群落。【方法】利用16S rRNA基因高通量测序技术分析对比原始接种污泥样品和驯化后阴极电极上生物膜样品多样性及结构变化。【结果】测序结果表明,原始接种污泥样品与驯化后阴极电极生物膜样品中微生物群落种类和结构存在显著差异,驯化后阴极电极生物膜样品中变形菌门(Proteobacteria)、γ-变形菌纲(Gammaproteobacteria)和特吕珀菌属(Trueperaceae)相对丰度比例高于原始污泥样品,成为优势菌群。【结论】驯化对系统阴极电极生物膜群落影响显著,随着产电量的输出,优势菌群不断富集,最终形成一个适应该实验环境下的新的微生物群落。对优势菌群结构和变化进行探讨,为生物阴极的研究补充更多生物学方面的理论基础。

[Background]Biocathode microbial fuel cell is a promising wastewater treatment system due to its low construction cost and sustainable development of the cathode.However,the oxidation-reduction properties of cathode microorganisms limit its further development in practical applications.[Objective]In order to improve the performance of the biocathode,it is necessary to understand the microbial community that affects the redox performance of the cathode.[Methods]The 16 S rRNA gene high-throughput sequencing technology was used to analyze and compare the microbial diversity and structural changes of the original inoculated sludge samples and the domesticated cathode electrode biofilm samples.[Results]The sequencing results showed that there were significant differences in the types and structures of microbial communities in the original inoculated sludge and the domesticated cathode electrode biofilm samples.The relative abundance ratios of Proteobacteria,Gammaproteobacteria and Trueperaceae in the cathodic electrode biofilm samples after domestication were higher than those of the original sludge samples,which became the dominant flora.[Conclusion]Domestication has a significant impact on the biofilm community of the cathode electrode of the system.With the output of electricity production,the dominant bacterial community is continuously enriched,and finally a new microbial community adapted to the experimental environment is formed.Discussing the structure and changes of the dominant flora,which provide more biological theoretical foundations for the research of biocathode.