摘要
为探究冷驯化条件下中缅树鼩(Tupaia belangeri)白色脂肪组织(WAT)和褐色脂肪组织(BAT)的差异代谢物变化,本研究采集对照组和冷驯化28天组中缅树鼩的WAT和BAT,采用非靶向代谢组液相色谱—质谱联用检测技术分析其差异代谢物含量变化。结果表明,冷驯化组较对照组WAT中有7种差异代谢物显著上调;BAT中有25种差异代谢物,其中23种差异代谢物显著上调,2种差异代谢物显著下调,主要参与三羧酸循环、脂类代谢、氨基酸代谢和糖代谢等。以上结果说明中缅树鼩通过利用脂肪组织的不同差异代谢物来抵御低温环境,提高生存率。
In order to investigate the changes of different metabolites of white adipose tissue(WAT)and brown adipose tissue(BAT)in Tupaia belangeri under cold acclimation,WAT and BAT were collected between control and cold acclimation group acclimated for 28 days in the present study,which were analyzed using non-targeted metabolome(liquid chromatography-mass spectrometer,LC-MS)detection technology.The results showed that 7 different metabolites were significantly up-regulated of WAT in cold acclimation group compared with that of control group;there were 25 different metabolites in BAT,among which 23 were up-regulated and 2 were down-regulated significantly,which were mainly involved in pathways of tricarboxylic acid cycle,lipid metabolism,amino acid metabolism and glucose metabolism.All of the above results indicated that T.belangeri could use different metabolites in different tissues to resist lower temperature and improve survival rate.
作者
陈思梦
贾婷
朱万龙
王政昆
CHEN Simeng;JIA Ting;ZHU Wanlong;WANG Zhengkun(Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College Kunming,School of Life Sciences of Yunnan Normal University,Engineering Research Center of Ministry of Education for Sustainable Development and Utilization of Bioenergy,Key Laboratory of Biomass Energy and Environmental Biotechnology in Yunnan Province,Kunming 650500,China;Yunnan College of Business Management,Kunming 650106,China)
出处
《兽类学报》
CAS
CSCD
北大核心
2021年第2期193-201,共9页
Acta Theriologica Sinica
基金
国家自然科学基金项目(31660121)
云南省中青学术和技术带头人后备人才项目(2019HB013)
云南省万人计划青年拔尖人才项目(YNWR-QNRC-2019-047)。
关键词
中缅树鼩
代谢组
脂肪组织
差异代谢物
冷驯化
Tupaia belangeri
Metabonomics
Adipose tissue
Differential metabolites
Cold acclimation
