滇重楼茎组织内生细菌的分离、鉴定及促生菌筛选

Isolation and identification of endophytic bacteria of Paris polyphylla var.yunnanensis stems and screening of growth-promoting strains

为了开发利用重楼内生细菌资源,从滇重楼茎组织中分离获得128株内生细菌,采用16S rDNA序列分析,将其鉴定归类到3个门,4个纲,6个目,11个科,14个属,其中芽孢杆菌属是优势属(75.0%),研究结果初步揭示了其茎组织内生细菌的多样性.进一步将128株内生细菌分别与小麦幼苗进行共培养,通过检测株高、根长、根数和鲜重等指标,筛选获得4株促生菌,分别是芽孢杆菌YNS21026和YNS32034、假单胞菌YNS21022和类芽孢杆菌YNS11005.其中芽孢杆菌YNS21026对小麦的株高和根长促进效果最好,增长率分别是87.2%和310%.该结果可为后期利用促生菌改良重楼种植技术提供菌种资源.

In order to develop and utilize endophytic bacteria of Paris polyphylla(P.polyphylla),128 endophytic bacteria were isolated from P.polyphylla var.yunnanensis stems and subsequently classified into 3 phyla,4 classes,6 orders,11 families,14 genera based on 16S rDNA sequence analysis.And among them,Bacillus was the dominant genus(75.0%).The results preliminary uncovered the diversity of endophytic bacteria in the P.polyphylla var.yunnanensis stems.All of the 128 endophytic bacteria were further co-cultured with wheat seedlings,respectively.Four growth-promoting endophytic bacteria were obtained through testing plant height,root length,root number,and fresh weight of the co-cultured wheat seedlings,which were Bacillus sp.YNS21026 and YNS32034,Pseudomonas sp.YNS21022 and Paenibacillus sp.YNS11005.The strain of Bacillus sp.YNS21026 had an obviously effect on the growth of plant height and root length of wheat seedlings,and the growth rates were promoted with 87.2%and 310%,respectively.These results provided microbial resources for improving the cultivation technology of P.polyphylla var.yunnanensis using growth-promoting bacteria.