徐州权台煤矿井下700m水平微生物群落分布特征

Distribution characteristics of microbial communities at a depth of 700 m level of Quantai coal mine in Xuzhou

煤矿井下与表生环境具有明显差异,研究其微生物群落分布及多样性对深入探讨井下水-岩-气-生作用机制具有重要意义。本研究在徐州权台煤矿井下700 m水平采集了8个沉积物样本,对样品进行了理化指标测试和Miseq高通量测序。结果表明,煤矿井下具有丰富的微生物多样性,8个样本共检测到35个细菌门类,其中变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)为优势菌门,其相对丰度之和占比超过80%;煤矿井下的优势菌属主要包括乳球菌属(Lactococcus)、假单胞菌属(Pseudomonas)、分枝杆菌属(Mycobacterium)和芽孢杆菌属(Bacillus)。从井下环境细菌群落分布来看,变形菌门和厚壁菌门主要分布在岩巷中,而放线菌门则主要分布在煤巷中。岩巷中的细菌多样性整体上高于煤巷。样本理化指标与微生物群落间的相关性分析表明,pH值是影响煤矿细菌群落门水平分布的主要因素(R2=0.766,P<0.05),其中放线菌、厚壁菌门和Latescibacteria与pH呈正相关,其他门类与pH呈负相关。TOC、总氮、总磷及硫酸盐对群落丰度分布也有一定的影响。因此,在煤矿污染机制及污染物迁移的研究中需充分考虑井下微生物的影响。

There are obvious differences between underground coal mine and surface environment.The studies of the distribution and diversity of microbial community in the underground coal mines are important for exploring the water-rock-gas-biological mechanism.In this study,eight coal deposit samples were collected from the level of-700 m in Quantai Coal Mine,Xuzhou,East China.The physical and chemical indices of the samples were tested and Miseq highthroughput sequencing was performed.A total of 35 bacteria phyla were detected in the eight samples,with Proteobacteria,Firmicutes,and Actinobacteria together accounting for more than 80%of the total abundance.Proteobacteria and Firmicutes were mainly distributed in rock lanes,while Actinobacteria were mainly distributed in coal lanes.The dominant bacterial genera of coal mines included Lactococcus,Pseudomonas,Mycobacterium,and Bacillus.In general,the abundance and diversity of bacterial communities in rock lanes were higher than those in coal lanes.pH was the key factor affecting the abundance of bacterial community in coal mine(R2=0.766,P<0.05)at the phylum level.pH was positively correlated with Actinobacteria,Firmicutes and Latescibacteria,while negatively correlated with other phyla.The concentrations of total organic carbon(TOC),total nitrogen(TN),total phosphorus(TP)and sulfate also affected the community abundance distribution.Therefore,the influence of underground microorganisms should be fully considered when examining coal mine pollution mechanism and pollutant migration.