超声波辅助提取长裙竹荪抑菌物质工艺优化及其抑菌活性研究

Study on the Technology Optimization and Antibacterial Activity of Ultrasonic-assisted Extraction of Antimicrobial Substances of Dictyophora indusiata

以长裙竹荪抑菌物质的抑菌效果为指标,采用抑菌圈法筛选出水、90%乙醇、乙酸乙酯3种提取剂中的最佳提取剂,再以最佳提取剂提取长裙竹荪抑菌物质,对胡萝卜软腐欧文氏菌、层出镰刀霉两种果蔬致腐菌和大肠杆菌做抑菌敏感性实验,筛选出最敏感菌种。结果表明,乙酸乙酯提取的长裙竹荪浸提液的抑菌效果最好,而且乙酸乙酯作为提取剂时,两种果蔬致腐菌均对长裙竹荪提取液表现出高度敏感,而大肠杆菌表现出低度敏感。最后以胡萝卜软腐欧文氏菌为目的菌,乙酸乙酯为提取剂,通过单因素和正交试验优化超声波辅助提取长裙竹荪抑菌物质的工艺。结果表明,各因素对长裙竹荪提取液抑菌效果的影响程度依次为:料液比>超声时间>超声功率,最终优化提取条件为:固液比1:20,超声功率560 W,超声时间40 min。

Take the antibacterial effects of antibacterial substances of Dictyophora indusiata as the indexes,the inhibition zone method is used to screen the best extractant from three extractants such as water,90%ethanol and ethyl acetate,afterwards,the optimal extractant is used to extract the antibacterial substances of Dictyophora indusiata,and the most sensitive bacteria are screened out by the test for two kinds of fruit and vegetable spoilage bacteria such as Erwinia carotovora and Fusarium proliferatum and Escherichia coli.The results show that the antibacterial effect of Dictyophora indusiata extraction solution extracted by ethyl acetate is the best,when ethyl acetate is used as the extractant,the two kinds of fruit and vegetable spoilage bacteria are highly sensitive to Dictyophora indusiata extraction solution,while Escherichia coli shows low sensitivity.Ultimately,the ultrasonic-assisted extraction process of antibacterial substances of Dictyophora indusiata is optimized by single factor and orthogonal tests,with Erwinia carotovora as the target bacterium and ethyl acetate as the extractant.The results show that the effects of various factors on the antibacterial effect of Dictyophora indusiata extraction solution are as follows:solid-liquid ratio>ultrasonic time>ultrasonic power,the final optimal extraction conditions are as follows:solid-liquid ratio is 1:20,ultrasonic power is 560 W,ultrasonic time is 40 min.