基于主成分和聚类分析的不同地区红花质量评价

Evaluation of the quality of safflower from different regions based on principal component and cluster analysis method

目的通过聚类分析,对不同产地红花的品质进行比较,为红花在陕西的培育及发展提供参考。方法采用UPLC法测定10批新疆红花与3批陕西红花中6-羟基山柰酚-3,6,7-三-O-葡萄糖苷、羟基红花黄色素A及脱水红花黄色素B的含量;并通过主成分分析及聚类分析对陕西与新疆不同产地的红花品质进行分析与评价。结果陕西省白水县及蒲城县的红花中6-羟基山柰酚-3,6,7-三-O-葡萄糖苷的含量最高,均为0.21%,新疆昌吉奇台县的红花中羟基红花黄色素A的含量最高,为1.93%,新疆米东区八家户村的红花中脱水红花黄色素B的含量最高,为1.10%,不同产地的红花质量存在一定差异,可以通过主成分分析及聚类分析将陕西与新疆地区的红花进行区分。结论红花中3种黄酮类成分可反映不同产地红花的内在质量,为不同产地红花的质量评价提供参考。

Objective To compare the quality of safflower from different producing areas through cluster analysis,and to provide references for the future cultivation and development of safflower in Shaanxi.Methods The contents of 6-hydroxy kaempferol-3,6,7-tri-O-glucoside,hydroxy safflower yellow A and dehydrated safflower yellow B in 10 batches of Xinjiang safflower and 3 batches of Shaanxi Province safflower were determined by UPLC,then,the quality of safflower from different producing areas in Shaanxi and Xinjiang was analyzed and evaluated by principal component analysis and cluster analysis.Results The safflowers in Baishui County and Pucheng County of Shaanxi province had the highest content of 6-hydroxy kaempferol-3,6,7-tri-O-glucoside(0.21%);the safflowers in Changji Qitai County of Xinjiang had the highest content of hydroxy safflower yellow A(1.93%);the safflowers in Eight Families Village of Midong District of Xinjiang had the highest content of dehydrated safflower yellow B(1.10%).There are certain differences in the quality of safflower from different producing areas in Shaanxi and Xinjiang,that can be distinguished by principal component analysis and cluster analysis.Conclusion The 3 flavonoids in safflower can reflect the internal quality of safflower from different producing areas,which provides a certain reference for the quality evaluation of safflower from different areas.