叉头框转录基因C1和上皮钙黏蛋白对肝癌临床预后及癌细胞生物学行为的影响

Effects of fork frame transcription gene C1 and epithelial Cadherin on clinical prognosis and biological behavior of liver cancer cells

目的探索叉头框转录基因C1(FOXC1)和上皮钙黏蛋白(E-Cadherin)表达水平与肝癌临床预后的关系,及其对肝癌细胞生物学行为的影响。方法用免疫组织化学法检测FOXC1和E-Cadherin在97例肝癌组织和及对应的癌旁组织标本中的表达,并分析两者表达的相关性。构建稳定干扰细胞系HepG2-shFOXC1,同时设置阴性对照HepG2-shNC和空白对照HepG2,用平板克隆实验和Transwell实验分别检测增殖和侵袭能力。结果在肝癌组织和癌旁组织中FOXC1蛋白阳性表达率分别为74.23%(72例/97例)和42.27%(41例/97例),E-Cadherin阳性表达率分别为29.89%(29例/97例)和100.00%(97例/97例),差异均有统计学意义(均P<0.05)。Spearman分析结果显示,FOXC1和E-Cadherin在肝癌组织中的表达呈负相关(P<0.01)。HepG2-shFOXC1组、HepG2组和HepG2-shNC组的克隆细胞数分别为(234.00±21.00),(897.00±89.00)和(898.00±84.00)个,通过matrigel基质胶的数量分别为(62.00±11.00),(171.00±15.00)和(167.00±17.00)个,HepG2-shFOXC1组的上述指标均明显低于HepG2组和HepG2-shNC组,差异均有统计学意义(均P<0.05)。结论FOXC1可能通过抑制E-Cadherin表达,从而增强肝癌细胞增殖和侵袭能力。

Objective To investigate the relationship between the expression levels of fork frame transcription gene C1(FOXC1)and epithelial Cadherin(E-Cadherin)on clinical prognosis of liver cancer,as well as the influence on the biological behavior of liver cancer cells.Methods Immunohistochemical method was used to detect the expression of FOXC1 and E-Cadherin in 97 cases of liver cancer tissues and their corresponding adjacent tissues,and the correlation between FOXC1 and E-Cadherin was analyzed.A stable interfering cell line HepG2-shFOXC1 was constructed,and negative control HepG2-shNC and blank control HepG2 were set at the same time.The proliferation and invasion abilities were detected by plate cloning test and Transwell test,respectively.Results The expression rates of FOXC1 protein in hepatocellular carcinoma and paracancerous tissues were 74.23%(72 cases/97 cases)and 42.27%(41 cases/97 cases),the expression rates of E-Cadherin protein were 29.89%(29 cases/97 cases)and 100.00%(97 cases/97 cases),respectively,with statistical significance(all P<0.05).Spearman analysis showed that there was negative correlation between FOXC1 and E-Cadherin expression in hepatocellular carcinoma(P<0.01).In HepG2-shFOXC1,HepG2 and HepG2-sh NC groups,the number of cloned cells were234.00±21.00,897.00±89.00 and 898.00±84.00,the number of cells passing through matrigel matrix glue were62.00±11.00,171.00±15.00 and 167.00±17.00,respectively.The above-mentioned indexes in HepG2-shFOXC1 group were significantly lower than those in HepG2 group and HepG2-sh NC group(all P<0.05).Conclusion FOXC1 may enhance the proliferation and invasion of hepatoma cells by inhibiting the expression of E-Cadherin.