基于16S rRNA技术研究电针对动脉粥样硬化兔动脉斑块及肠道菌群科水平的影响

Effects of electroacupuncture on arterial plaque and intestinal flora at family level in atherosclerosis rabbits based on 16S rRNA technology

目的:观察电针防治干预对动脉粥样硬化(AS)动脉斑块及科水平肠道菌群结构特征的影响,筛选特征性菌群,探析电针抗AS的机制。方法:实验兔平均随机分为空白组、模型组、防治组,每组6只。模型组以高脂饲料结合颈总动脉球囊损伤术方法造模;防治组先予电针干预后造模,后再予电针干预。观察油红O染色、16S rRNA测序与生信分析结果。结果:防治组颈动脉内壁沉积脂质斑块明显少于模型组;空白组与防治组组间肠道菌群群落差异更小;防治组S24-7、Synergistaceae、Veillonellaceae、[Mogibacteriaceae]科菌群相对丰度与模型组比较,差异有统计学意义(P<0.05,P<0.01),与空白组相近。结论:AS与肠道菌群结构变化有关,电针能抑制动脉斑块形成并调整肠道菌群结构,可能与S24-7 Veillonellaceae、[Mogibacteriaceae]科菌群相对丰度有关。

Objective: To observe the characteristics of arterial plaque and family level of intestinal flora in atherosclerosis(AS), rabbits after electroacupuncture preventive and treatment intervention, screen mark intestinal flora, and explore the prevention mechanism of electroacupuncture on AS. Methods: Experimental rabbits were randomly and average divided into blank group, model group and preventive group,6 rabbits each group. The model group was fed with high-fat diet combined with common carotid balloon injury. The preventive group firstly received electroacupuncture intervention. Then, the preventive group was modeled in the same way as the model group, and finally intervened with electroacupuncture. After the intervention, oil red O staining and 16 S rRNA high-throughput sequencing were performed. Results: Compared with the model group, there were fewer atherosclerosis plaques deposited on the carotid wall in the preventive group;the difference of the microbial community between the blank group and the preventive group was smaller;S24-7, Synergistaceae, Veillonellaceae, and [Mogibacteriaceae] bacterial flora abundance in preventive group were significantly different from the model group(P<0.05, P<0.01), and were similar to the blank group. Conclusion: AS is related to changes of intestinal flora structure. Electroacupuncture can effectively inhibit the formation of arterial plaques and adjust the structure of intestinal flora, probably through regulating the abundance of S24-7, Veillonellaceae, and [Mogibacteriaceae].