四翅滨藜AcDREB2转录因子编码基因的克隆及其表达

Cloning and expression of the AcDREB2 transcription-factor gene in Atriplex canescens

脱水应答元件结合蛋白(DREB)在植物响应盐和干旱胁迫中发挥重要作用。为研究DREB在耐盐碱耐干旱的先锋植物四翅滨藜(Atriplex canescens)中的功能及其应用前景,本研究设计了一对简并引物,通过PCR扩增和RACE法克隆得到四翅滨藜DREB转录因子编码基因AcDREB2。该基因cDNA全长为867 bp,共编码242个氨基酸。序列BLAST比对与同源性分析结果表明,该基因与其他植物的DREB具有较高的同源性。利用RT-PCR方法进行表达模式分析发现,AcDREB2在四翅滨藜叶中高丰度表达,且其表达受NaCl和渗透胁迫处理的快速诱导,表明AcDREB2参与了四翅滨藜的抗逆响应。

Dehydration-responsive element-binding proteins(DREB)play an important role in plant responses to salt and drought stress.To investigate the function of DREB in Atriplex canescens and their application prospects,a pair of degenerate primers was designed,and the DREB transcription-factor coding-gene AcDREB2 was cloned via PCR(polymerase chain reaction)amplification and the RACE(Remote Analysis Computation for gene Expression data)method.The full-length cDNA of AcDREB2 was 867 bp,encoding 242 amino acids.The BLAST and homology matrix results showed that AcDREB2 was highly homologous with the DREBs of other plants.The expression patterns of AcDREB2 were analyzed via RT-PCR.AcDREB2 was highly expressed in the leaves of A.canescens,and its expression was rapidly induced by NaCl and osmotic stress,indicating that AcDREB is involved in the A.canescens stress response.