种植体周围炎大鼠模型研究

Modification of Rat Model with Peri-implantitis

目的:改良大鼠种植体周围炎模型,简化造模步骤,缩短造模时间。方法:拔除16只大鼠上颌第一磨牙,拔牙窝愈合1个月后植入钛种植体。种植体骨结合4周后用丝线结扎诱导炎症并糖水喂养。结扎后2周观察炎症诱导情况。处死大鼠,取上颌骨拍摄X线片用于影像学分析。制作骨组织切片和提取种植体周围牙龈组织进行免疫组化分析。结果:结扎2周后种植体周围炎诱导成功,种植体周围不仅有软组织炎症,还有不同程度骨组织吸收。结论:大鼠种植体周围炎模型改良成功,造模方便,造模周期短。

Objective:To simplify the steps and shorten the time of reconstruction of rat peri-implantitis model.Methods:The maxillary first molars of 16 rats were extracted,and titanium implants were implanted one month later.After 4 weeks,silk thread ligation was used to induce inflammation and the rats were fed with sugar water.Two weeks later,the rats were sacrificed,the maxilla was taken X-rays for imaging analysis,bone tissue slices were made,and the gingival tissue around the implant was extracted for immunohistochemical analysis.Results:Two weeks after ligation,peri-implantitis was successfully induced.There was not only soft tissue inflammation around the implant,but also bone tissue resorption at various degrees.Conclusion:The model of rat peri-implantitis has been successfully constructed,with convenience and short modeling cycle.