香叶木苷联合华法林治疗深静脉血栓机制的网络药理学分析

Network Pharmacology Analysison the Mechanism of Diosmin Combined with Warfarin in the Treatment of Deep Vein Thrombosis

目的:基于网络药理学探讨香叶木苷联合华法林治疗深静脉血栓的分子机制。方法:以Swiss Target Prediction数据库获取香叶木苷及华法林作用靶点,OMIM和GenCard s数据库获取深静脉血栓形成的靶点,通过绘制韦恩图发现香叶木苷及华法林治疗深静脉血栓的潜在作用靶点,并建立靶点蛋白相互作用网络。于Metascape数据库进行GO功能和KEGG通路分析。结果:经Swiss Target Prediction预测得到24个香叶木苷潜在作用靶点、96个华法林潜在作用靶点。经网络分析可知,香叶木苷及华法林主要作用于AKT1、TNF、MAPK1等靶蛋白,KEGG富集分析得到香叶木苷及华法林主要作用于PI3K-AKT信号通路和表皮生长因子受体酪氨酸激酶抑制剂耐药通路。结论:香叶木苷与华法林联合用药可能通过多靶点相互作用方式,干预多条信号通路,进而调控血栓形成和发展。

Objective:To analysis the molecular mechanism of diosmin combined with warfarin in the treatment of deep vein thrombosis based on network pharmacology.Methods:The potential targets of diosmin and warfarin through the Swiss Target Prediction database,and the targets of deep vein thrombosis were obtained from the OMIM and GenCards databases.The Venn diagram was drawn to discover the potential targets of diosmin and warfarin in the treatment of deep vein thrombosis,and the target protein interaction network was established.GO function and KEGG pathway analysis were performed in Metascape database.Results:24 potential targets of diosmin and 96 potential targets of warfarin were predicted by Swiss Target Prediction.Network analysis showed that diosmin and warfarin mainly acted on target proteins such as AKT1,TNF,and MAPK1.KEGG enrichment analysis showed that diosmin and warfarin mainly acted on PI3K-AKT signaling pathway and EGFR tyrosine kinase inhibitor resistance.Conclusion:The combination of diosmin and warfarin may intervene in multiple signaling pathways through multi-target interactions,thereby regulateformation and development of thrombosis.