ssc-miR-17-3p调控脂肪沉积的靶基因生物信息学分析

Bioinformatics analysis of target genes regulated by ssc-miR-17-3p in fat deposition

【目的】找到与猪脂肪沉积相关的候选靶基因,为ssc-miR-17-3p参与脂肪沉积调控机制研究提供相关理论依据。【方法】利用miRDB,miRWalk和TargetScan数据库获得了miRNA-17-3p候选靶基因;利用DAVID和KOBAS数据库对候选靶基因进行了KEGG/GO功能富集分析,并通过Cytoscape可视化工具构建了其可能参与的调控网络。【结果】结果表明,ssc-miR-17-3p候选靶基因TSTD2、CSDE1、SCGB1D1、TEAD1、GPD2、NFATC3、MBNL1、PPP1R12B、CAMK2D、HBEGF、LRRC28、UBE2D4、MAP4与脂肪沉积有关。GPD2、CAMK2D、HBEGF等可能在催产素信号通路、MAPK信号通路、GnRH信号通路,以及脂质代谢、甘油磷脂代谢、糖醇代谢、大分子代谢,细胞代谢等过程发挥了作用。【结论】ssc-miR-17-3p可能通过调控多个靶基因,信号通路和生物过程影响脂肪的沉积与代谢,其功能有待进一步实验室试验验证。

[Objective]Finding candidate target genes related to pig fat deposition provides a theoretical basis for the study of ssc-miR-17-3 p involved in the regulation of fat deposition.[Methods]The candidate target gene of miRNA-17-3 p was obtained by using miRDB,miRWalk and TargetScan database;The KEGG/GO functional enrichment analysis of candidate target genes was carried out by using DAVID and KOBAS database,and the regulatory network that they might participate in was constructed by Cytoscape visualization tool.[Results]The results showed that the candidate target genes of ssc-miR-17-3 p,including TSTD2,CSDE1,SCGB1 D1,TEAD1,GPD2,NFATC3,MBNL1,PPP1 R12 B,CAMK2 D,HBEGF,LRRC28,UBE2 D4 and MAP4,were associated with fat deposition.GPD2,CAMK2 D and HBEGF might play an important role in oxytocin signal pathway,MAPK signal pathway,GnRH signal pathway,as well as lipid metabolism,glycerophospholipid metabolism,alditol metabolic process,macromolecular metabolism,cell metabolism and so on.[Conclusion]ssc-miR-17-3 p might affect fat deposition and metabolism by regulating multiple target genes,signal pathways and biological processes,but its function needs to be verified by further laboratory experiments.