摘要
[Objectives]Ultraviolet spectrophotometry was established for the determination of total content of anthraquinone in different processed products of rhubarb.[Methods]Emodin was used as the control substance,and its color was developed by magnesium acetate methanol solution with the concentration of 1%,the absorbance was determined at the wavelength of 516 nm,the content of total anthraquinone in different processed products of rhubarb was determined to compare the content differences.[Results]The regression equation was y=0.0452 x+0.0015,R 2=0.9991.Emodin had a good linear range in the concentration of 0.98-23.52μg/mL,with high accuracy,good repeatability and stable detection results.This method revealed the content changes of total anthraquinone in rhubarb before and after processing.[Conclusions]This method could be used as the quality control method of different processed products of rhubarb,and provided reference for the comprehensive development and utilization of rhubarb resources in the future.
基金
Supported by Youth Fund Project of Guangxi University of Chinese Medicine(2019QN036)
National Talent Training Program for Inheriting Characteristic Technology of Traditional Chinese Medicine(20184828005)
Guangxi Natural Science Foundation Project(2020GXNSFAA259059)
The Project of Improving the Basic Scientific Research Ability of Young-and Middle-aged Teachers in Guangxi Universities in 2019(2019KY0341)
In Hospital Preparation Development Project of the First Affiliated Hospital of Guangxi University of Chinese Medicine in 2017(2017ZJ001)
Opening Project of Guangxi Zhuang Yao Medicine Key Laboratory(GXZYKF2020A-08)
Scientific Research Projects of Guangxi International Zhuang Medicine Hospital in 2020(GZ202001).
