近部与远部穴位埋线对原发性痛经大鼠子宫前列腺素、血清IL-2及脾脏NK细胞的影响

Effect of proximal and distal acupoint catgut-embedding on uterus prostaglandin, serum IL-2 and splenic NK cell activity in primary dysmenorrhea rats

目的:观察近部与远部穴位埋线对原发性痛经(PD)大鼠子宫组织前列腺素E2(PGE2)和前列腺素F2α(PGF2α)含量、血清白细胞介素-2(IL-2)含量、脾脏自然杀伤(NK)细胞活性的影响,探讨近部与远部穴位埋线对PD大鼠的效应差异。方法:雌性Wistar大鼠随机分为对照组、模型组、近部埋线组、远部埋线组,每组8只。皮下注射苯甲酸雌二醇(第1天和第10天按0.5 mg/只注射,第2~9天按0.2 mg/只注射)联合腹腔注射缩宫素(末次苯甲酸雌二醇给药24 h后,2 U/只)制备PD大鼠模型。近部埋线组选取"关元""次髎"埋线治疗,远部埋线组选取"三阴交""地机"埋线治疗,均于造模前1天进行。造模结束后,比较各组大鼠30 min内扭体次数,子宫质量,ELISA法测定子宫组织PGE2、PGF2α含量及血清IL-2含量,MTT比色法检测脾脏NK细胞活性。结果:与对照组比较,模型组大鼠扭体次数增多(P<0.01);与模型组比较,近部、远部埋线组扭体次数均减少(P<0.01)。与对照组比较,模型组大鼠子宫质量增加(P<0.01),子宫组织PGE2含量降低(P<0.01)、PGF2α含量升高(P<0.01),血清IL-2含量降低(P<0.01),脾脏NK细胞活性降低(P<0.01);与模型组比较,近部、远部埋线组子宫质量均降低(P<0.01,P<0.05),子宫组织PGE2含量升高(P<0.01)、PGF2α含量降低(P<0.01),血清IL-2含量升高(P<0.01),脾脏NK细胞活性升高(P<0.01)。近部埋线组较远部埋线组PGF2α含量降低(P<0.05),脾脏NK细胞活性升高(P<0.01)。结论:近部穴位埋线与远部穴位埋线均可提高PD大鼠子宫PGE2含量、血清IL-2含量及脾脏NK细胞活性,降低子宫PGF2α含量,从而达到镇痛效果。且近部取穴治疗一定程度上要优于远部取穴。

Objective To observe the effect of proximal and distal acupoint catgut-embedding on the contents of prostaglandin E2(PGE2) and prostaglandin F2α(PGF2α) in the uterus, serum Interleukin-2(IL-2) contents and splenic natural killer(NK) cell activity in primary dysmenorrhea(PD) rats, so as to explore the different effects of proximal and distal acupoint catgut-embedding on PD rats. Methods Female Wistar rats were randomly divided into control, model, proximal catgut-embedding and distal catgut-embedding groups, with 8 rats in each group. The PD model was established by subcutaneous injection of estradiol ben-zoate(0.5 mg/rat on the first day and 10th day, and 0.2 mg/rat from the 2nd to the 9th day) and intraperitoneal injection of oxytocin(2 U/rat, on the 11th day). Catgut-embedding was applied at bilateral "Guanyuan"(CV4) and "Ciliao"(BL32) in the proximal catgut-embedding group, and bilateral "Sanyinjiao"(SP6) and "Diji"(SP8) in the distal catgut-embedding group. After the treatment, the body writhing times in 30 min and uterine mass were recorded, PGE2 and PGF2αin uterus and IL-2 in serum were assayed by using ELISA, the activity of NK cell in the spleen was detected using MTT colorimetry. Results Following modeling, the body writhing times of the model group was increased than that of the control group(P<0.01);After interventions, the body writhing times were decreased in the proximal and distal catgut-embedding groups than those of the model group(P<0.01). Compared with the control group, the uterine mass and uterus PGF2αcontent were increased(P<0.01), while uterus PGE2, serum IL-2 and splenic NK cell activity were decreased(P<0.01) in the model group. After interventions, the uterine mass and uterus PGF2α were down-regulated(P<0.01, P<0.05), while the contents of uterus PGE2, serum IL-2 and splenic NK cell activity up-regulated(P<0.01) in the proximal and distal catgut-embedding groups in contrast to the model group. The content of uterus PGF2α was down-regulated(P<0.05) and splenic NK cell activity was up-regulated(P<0.01) in the proximal catgut-embedding group than those of the distal catgut-embedding group. Conclusion Both proximal and distal acupoint catgut-embeding can increase PGE2 and decrease PGF2α in the uterus, increase the level of serum IL-2 and the activity of NK cells in the spleen in PD model rats, thereby achieving analgesic effect. The effect of proximal catgut-embedding is better than that of distal catgut-embedding.