2019年湖北省首起登革热本地感染疫情病原分子溯源

Molecular traceability of the pathogen of the first local dengue infection outbreak in Hubei Province in 2019

目的分离培养及鉴定湖北省2019年首起本地感染登革热病例病原体,以确定病毒的血清型和基因型并进行溯源。方法采集患者血清标本用酶联免疫吸附试验(ELISA)进行IgM和IgG抗体检测;用荧光定量PCR方法鉴定登革热病毒血清型;采用C6/36细胞分离登革热病毒后获取病毒E基因和全基因组序列进行系统进化分析,追溯可能的感染来源。结果该起疫情病原鉴定为登革热病毒血清Ⅰ型并分离到登革热Ⅰ型毒株5株;经系统进化分析属于登革热Ⅰ型中的基因Ⅰ型,与2019年广州分离株同源性最高。结论湖北省2019年首起登革热本地感染疫情由Ⅰ型登革热病毒的基因Ⅰ型引起。

Objective To identify the etiology of the first local dengue infection outbreak in Hubei Province in 2019,and to determine the serotype and genotype of the virus and trace its source.Methods Serum samples were collected from dengue fever cases in the acute phase.The IgM and IgG antibodies were detected by enzyme-linked immunosorbent assay(ELISA),and the serotype was determined by fluorescence quantitative PCR.C6/36 cells were used to isolate virus and obtain virus E gene and complete genome sequence for systematic evolution analysis to trace the possible source of infection.Results The pathogen of the outbreak was identified as dengue serotype I infection,and five virus strains were isolated.Sequence analysis showed that the virus belonged to genotype I of dengue I,and had the highest homology with the strain isolated in Guangzhou,2019.Conclusion The first local dengue infection outbreak in Hubei Province in 2019 was caused by genotype I of the type I dengue virus.