生物信息学软件预测猪氨基肽酶N功能与结构

Prediction of Function and Structure of Porcine Aminopeptidase N by Using Bioinformatic Software

[目的]研究猪δ冠状病毒(PDCoV)入侵宿主细胞的感染机制,对其特异性受体猪氨基肽酶N基因编码蛋白功能与结构进行分析。[方法]通过多种生物信息学软件对pAPN蛋白的理化特性、亲疏水性、信号肽、跨膜区、糖基化位点、抗原表位、功能结构域及结构特征等进行预测分析。[结果]pAPN基因总长为2892 bp,编码963个氨基酸;其中,第35~963位氨基酸为膜外蛋白区,不含信号肽但包含2个典型的功能结构域。pAPN蛋白共有23个糖基化修饰位点和42个抗原表位。pAPN蛋白胞外域空间结构为二聚体,每个单体均可分为Ⅰ~Ⅳ4个结构域。[结论]该研究为了解pAPN蛋白与多种猪肠道冠状病毒相互作用的机制提供了思路。

[Objective]To investigate the underlining molecular mechanism of porcine deltacoronavirus(PDCoV)invading the host cells,the structure and function of the protein that served as a specific receptor of PDCoV and encoded by the aminopeptidase N gene(pAPN)was bioinformatically analyzed.[Method]A variety of bioinformatic software was used to predict and assess the physical and chemical properties,hydrophilicity and hydrophobicity,signal peptide,transmembrane domain,glycosylation site,antigenic epitope,functional domain,and structural characteristics of pAPN protein.[Result]The total length of pAPN gene is 2892 bp,which encodes 963 amino acids.Among them,the 35th~963th amino acids are the extramembrane protein region,which do not contain signal peptide but contain two typical functional domains.pAPN protein contains 23 glycosylation modification sites and 42 antigenic epitopes.The spatial structure of extracellular domain of pAPN protein is a dimer,and each monomer can be divided into four domains ofⅠ~Ⅳ.[Conclusion]This study provides information for understanding the molecular mechanism of interactions between pAPN protein and various porcine enteric coronaviruses.