病毒感染时IFITM3与TRIM22和Foxp3表达及关联基因的富集分析

Relative expression of the IFITM3 and TRIM22 genes and enrichment analysis of related genes during infection with three viruses

目的病毒感染时对IFITM3、TRIM22和FOXP3及基因进行富集分析。方法利用STRING作出IFITM3、TRIM22和FOXP3及其关联的50个基因互作网络图;利用DAVID数据库和KOBAS数据库对其进行GO分析和KEGG分析。应用荧光定量PCR对3基因的mRNA相对表达量进行相关性分析。结果通过STRING数据库分析得到3基因互作网络图,关联性大小与图中代表基因节点的圆圈大小呈正比(P<0.05)。经基因功能富集分析表明基因相关的信号通路与基因有关联的可信度较高(P<0.05)。在乙型肝炎病毒、丙型肝炎病毒和甲型流感病毒感染患者的外周血白细胞(P<0.05)分别为(4.462±3.441)、(4.406±4.415)和(4.013±0.081)中的IFITM3的mRNA与健康对照组差异有统计学意义(t=2.987,P<0.01)。结论机体感染病毒后,IFITM3与TRIM22之间、FOXP3与TRIM22之间可能存在着一定的相互作用,并参与机体对病毒感染的应答进程。

Objectives To perform enrichment analysis of IFITM3,TRIM22,FOXP3,and related genes during virus infection.Methods The STRING database was used to map the interaction network for IFITM3,TRIM22,FOXP3,and 50 related genes.The DAVID and KOBAS databases were used to perform GO analysis and KEGG analysis of these genes and to identify their related signal pathways and routes of viral infection.The relative expression of the 3 genes was analyzed using RT-PCR.Results The gene interaction network for IFITM3,TRIM22,and FOXP3 was mapped based on an analysis of the STRING database,and the size of the correlation was proportional to the size of the circle representing the gene node in the figure(P<0.05).Gene function enrichment analysis indicated that influenza A,hepatitis C,and hepatitis B pathways are all related to genes in gene-related signaling pathways with a high level of reliability(P<0.05).The signaling pathway with the highest degree of correlation to three genes is the T cell receptor signaling pathway.IFITM3 mRNA was 4.462±3.441 in peripheral blood leukocytes(P<0.05)of patients infected with hepatitis B virus,4.406±4.415 in patients infected with hepatitis C virus,and 4.013±0.081 in patients infected with influenza A virus.The difference in mRNA levels between patients and the healthy control group was significant(t=2.987,P<0.01).Conclusion There may be some interaction between IFITM3 and TRIM22 and between FOXP3 and TRIM22 that may be involved in the response to a viral infection.