猕猴桃抗溃疡病转录组分析和基因功能注释

Transcriptome Analysis and Gene Function Annotation of Kiwifruit Psa Resistance

为了发掘中国野生猕猴桃资源抗溃疡病基因,利用Illumina HiSeq测序平台对不同时间接种溃疡病菌的毛花猕猴桃进行mRNA高通量测序。结果表明,共获得68731个Unigene,其中有48414个基因注释到Nr、SwissProt、KEGG和COG/KOG数据库。KOG注释显示,通用功能预测、转录后修饰、蛋白代谢、伴侣蛋白、信号转导机制,翻译、核糖体结构和生物合成所占比例最高;GO注释表明参与生物过程的差异表达基因数目最多,细胞组分和分子功能次之;KEGG通路分析表明差异基因的富集以生物合成和代谢为主。接种后不同时间点共获得63050个差异表达基因。通过SSR位点分析,从68731个Unigene中鉴定出13652个SSRs位点;同时获得了各类型转录因子1580个;R基因3727个。本研究结果对猕猴桃抗溃疡病基因发掘和抗溃疡病新品种选育具有重要的理论指导和育种实践意义。

In order to find the Psa resistance gene of Chinese wild kiwifruit,the Illumina HiSeqTMplatform was used to analyze the m RNA of Actinidia eriantha at different times after Psa inoculation.As a result,68731 Unigenes were acquired and 48414 Unigenes were annotated in Nr,SwissProt,KEGG and COG/KOG database.The annotation of KOG revealed that general function prediction,posttranslational modification,protein turnover,chaperones,signal transduction mechanisms,translation,ribosomal structure and biogenesis were much higher than others.GO annotation showed that the largest group was biological,followed by cellular component and molecular function.The biosynthesis and metabolism pathways were most highest in KEGG pathways.There were63050 DEGs(differential expressed genes)in different times after inoculation.A total of 13652 SSR loci were detected in 63781 Unigenes.And 1580 transcription factors and 3727 R genes were obtained.The result is important for studying Psa-resisting genes and breeding of Psa-resisting kiwifruit.