黄芩等6味中药对多重耐药鲍曼不动杆菌的体外抑菌活性的实验研究

Experimental Study on Antibacterial Activity of Scutellariae Radix and Other 5 Kinds of TCM Against Multi-drug Resistant Acinetobacter Baumannii in Vitro

目的:探究黄芩、黄连等6味中药材对多重耐药鲍曼不动杆菌(MDR-AB)的体外抑菌和杀菌活性。方法:选用黄芩、黄连、连翘、乌梅、穿心莲、金银花药材的提取物,采用药敏纸片琼脂扩散法及肉汤稀释法,测定提取物对MDR-AB的体外抑菌圈的最低抑菌浓度(MIC)。结果:黄芩、黄连提取物有较强的抑菌活性,抑菌圈直径大,MIC值较低;而乌梅提取物有一定的抑菌、杀菌活性,连翘、穿心莲、金银花提取物的抑菌活性相对较弱。结论:6味中药材的提取物对MDR-AB均有不同程度的抑菌活性,其中黄芩、黄连、乌梅3种提取物对10株MDR-AB的活性相对较高,而黄芩的抑菌效果为最好。

Objective:To explore the antibacterial and bactericidal activities of Scutellariae Radix and Rhizoma Coptidis and Other 5 kinds of TCM against multi-drug resistant Acinetobacter Baumannii in Vitro(MDR-AB). Methods:The extracts of Scutellariae Radix, Rhizoma Coptidis, Fructus Forsythiae, Forsythia Fructus, Lonicera Japonica Flos and Andrographis Herba were selected, and the minimum inhibitory concentration(MIC) of the extracts to the in Vitro inhibitory zone of MDR-AB was determined using drug-sensitive paper AGAR diffusion method and broth dilution method. Results:The extracts of Scutellariae Radix and Rhizoma Coptidis had strong inhibitory activity, large inhibitory circle diameter and low MIC value. The extracts of Fructus Forsythiae had some antibacterial and bactericidal activities,while the extracts of Forsythia Fructus, Lonicera Japonica Flos and Andrographis Herba had relatively weak antibacterial activities. Conclusion:The extracts of 6 kinds of TCM all had different degree of antibacterial activity against MDRAB, among which the 3 extracts of Scutellariae Radix, Rhizoma Coptidis and Fructus Forsythiae had relatively high activity against 10 strains of MDR-AB,and the antibacterial effect of Scutellariae Radix was the best.