复心汤治疗心衰大鼠的线粒体通透性转换孔机制研究

Mechanism of Mitochondrial Permeability Transition Hole in Rats with Heart Failure Treated by Fuxin Decoction(复心汤)

目的观察复心汤治疗心衰大鼠的疗效及与线粒体通透性转换孔(mitochondrial permeability transition pore,mPTP)开放程度的相关性,揭示复心汤治疗心衰的mPTP机制。方法建立空白对照组、心衰模型组、西药对照组、复心汤组4个试验组,每组20只大鼠。采用阿霉素腹腔注射Wistar大鼠建立心力衰竭模型。按干预方法处理4周后,比较各组大鼠死亡率、血清BNP值,心脏彩超评测心功能情况。测定各组大鼠心肌线粒体在mPTP开放诱导剂作用下A540吸光度的改变,以评测心肌线粒体mPTP的开放程度。观察复心汤组各疗效数值与心肌线粒体mPTP开放程度的相关性。结果复心汤组与模型组和西药对照组相比,各项指标均有显著改善,心脏彩超LVEDD、LVEDS较两组明显减小,FS、EF较两组明显增大,BNP、死亡率较两组明显降低,mPTP开放诱导剂作用下线粒体A540吸光度的改变显著增大,并且复心汤组线粒体A540吸光度变化程度与LVEDS等观察指标具有相关性。结论复心汤能改善mPTP通透性,可能因此改善了心肌线粒体能量代谢及心肌凋亡情况,使复心汤取得疗效。

Objective To observe the relationship between the therapeutic effect of Fuxin Decoction(复心汤)and the opening degree of mitochondrial permeability transition pore(mitochondrial permeability transition pore,mPTP)in rats with heart failure and explain the mechanism of Fuxin Decoction treating heart failure.Methods The blank control group,heart failure model group,western medicine control group and Fuxin Decoction group were established with 20 rats in each group.The model of heart failure was established by intraperitoneal injection of adriamycin into Wistar rats.After 4 weeks of intervention,the mortality rate and plasma BNP value were estimated.The cardiac function was measured by color Doppler echocardiography and the changes of A540 absorbance of myocardial mitochondria under the action of mPTP open inducer were measured to evaluate the degree of mPTP opening of myocardial mitochondria in each group.We observed the correlation between the measured values of Fuxin Decoction group and the opening degree of myocardial mitochondrial permeability transition pore.Results Compared with the model group and the western medicine control group,the indexes of Fuxin Decoction group were significantly improved,the levels of LVEDD and LVEDS of heart color Doppler ultrasound were lower than those of the two groups.The levels of FS and EF of the two groups were obviously increased,and the death rate and BNP were lower than those of the two groups.The changes of A540 absorbance of mitochondria were significantly increased under the action of mPTP open inducer and the degree of A540 absorbance change in Fuxin Decoction group was correlated with LVEDS and other indexes.Conclusion Fuxin Decoction can improve the permeability of mPTP,which may improve the energy metabolism of myocardial mitochondria and myocardial apoptosis and so that the curative effect can be obtained.