滞育期九香虫卵巢组织蛋白的LC-MS/MS鉴定

Identification of Ovarian Tissue Proteins in the Diapause Period of Aspongopus Chinensis Dallas by Using LC-MS/MS

九香虫的越冬滞育是影响其大规模人工繁殖的桎楛,保幼激素可调控九香虫的滞育,并可促进卵巢发育。为探索滞育期九香虫卵巢组织蛋白的组成,本研究提取滞育期与滞育解除期的九香虫卵巢组织蛋白,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行初步分析,液相色谱-串联质谱法(LC-MS/MS)对胰酶消化产生的肽段混合物进行分析。结果表明,质谱共检测到448条肽段,唯一肽段为341条,分布于205种蛋白中,分子量集中在10~60 kDa,其中,保幼激素结合蛋白、过氧化氢酶、ATP酶、精氨酸激酶、热休克蛋白及相关同源蛋白的表达可能与九香虫滞育期的越冬抗逆性、调控生长发育、能量代谢及维持滞育有关。

The overwintering diapause of Aspongopus chinensis Dallas affected their large-scale artificial reproduction,juvenile hormone could regulate the diapause of of A.chinensis and promote the development of ovarian.In order to explore the composition of the ovarian tissue proteins during the diapause period,this study extracted the ovarian tissue proteins during the diapause and diapause termination period.Sodium dodecyl sulphate-polyacrylamide gel(SDS-PAGE)was used for electrophoresis analysis,and the peptides digested by trypsin were analyzed by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The results showed that mass spectrometry detected a total of 448 peptide,the number of unique peptide was 341,which were distributed in 205 kinds of proteins,and the molecular weight of these proteins ranged from 10 to 60 kDa.The expression of juvenile hormone binding protein,catalase,ATPase,arginine kinase,heat shock protein and related homologous proteins may be related to the resistance to overwintering,growth,development,energy metabolism and maintenance of diapause in A.chinensis.