大白菜细菌性角斑病抗性鉴定方法的建立

Establishment of Identification Method for Resistance to Bacterial Angular Leaf Spot in Chinese Cabbage

近年来华北和东北地区大白菜细菌性角斑病的危害逐年加重,抗角斑病已成为大白菜育种的重要研究方向,但目前尚未建立完善的大白菜抗细菌性角斑病鉴定方法。本试验对哈尔滨市向阳乡东北农业大学实验实习基地大白菜细菌性角斑病病原菌进行分离鉴定,确定病原菌为丁香假单孢菌丁香致病型(Pseudomonas syringae pv.syringae van Hall)。以该分离物为接种病原,以大白菜自交系A224-1为试验材料,采用L_(9)(3^(4))正交试验设计,分析接种温度、湿度、苗龄对细菌性角斑病发病的影响;并以自交系264-1-1、203-1及331-1为试验材料,筛选适宜的接种浓度。结果表明:苗龄7~8叶期、接种温度25~30℃、接种湿度90%~100%为大白菜细菌性角斑病发病的最佳条件组合,最佳接种浓度为1×10^(10) cfu·mL^(-1)。

Recently damages brought by bacterial angular leaf spot to Chinese cabbage in north and northeastern China has been aggravated year by year.Breeding cultivars resistant to angular leaf spot has become an important research direction for breeding of Chinese cabbage.But until now a complete identification method for Chinese cabbage resistance to angular leaf spot has not yet been established.This experiment carried out isolation and identification on pathogen of bacterial angular leaf spot in Chinese cabbage planted at the experiment and practice base of Northeast Agricultural University,and confirmed this pathogen was Pseudomonas syringae pv.syringae van Hall.Taking this isolate as inoculating pathogen and Chinese cabbage inbred line A224-1 as test material,this experiment adopted orthogonal experiment design of L_(9)(3^(4)),analyzed the effects of inoculation temperature,inoculation humidity and seedling age on occurrence of bacterial angular leaf spot.The experiment also took inbred line 264-1-1,203-1 and 331-1 as test material to screen out appropriate inoculating concentration.The results showed that the optimal condition combination for occurrence of bacterial angular leaf spot in Chinese cabbage was 25-30℃ inoculation temperature 90%-100%inoculation humidity 7-8 leaf-stage seedling age,and optimal inoculation concentration was 1×10^(10) cfu·mL^(-1).