薏苡仁提取物经PDGFB/PDGFRβ信号通路对口腔鳞癌细胞增殖、迁移和侵袭的影响

Effect of semen coicis extract on proliferation,migration and invasion of oral squamous cell carcinoma cells via PDGFB/PDGFRβsignal pathway

目的观察薏苡仁提取物(ESC)经血小板源性生长因子B(PDGFB)/血小板源性生长因子受体β(PDGFRβ)信号通路对口腔鳞癌细胞增殖、迁移和侵袭的影响。方法2020年6—8月于甘肃省人民医院实验室进行实验。体外培养人口腔鳞癌CAL27细胞,设对照组(不予处理)、ESC低剂量组(10μmol/L)、ESC高剂量组(20μmol/L)和伊马替尼组(10μmol/L),培养24 h后检测细胞增殖率、迁移能力和侵袭能力,同时采用蛋白免疫印迹法检测PDGFB、PDGFRβ、磷酸化PDGFRβ(p-PDGFRβ)、磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(Akt)和磷酸化Akt(p-Akt)蛋白表达水平。结果与对照组比较,ESC低剂量组、ESC高剂量组及伊马替尼组CAL27细胞增殖率、迁移能力、细胞侵袭数及PDGFB、PDGFRβ、p-PDGFRβ、PI3K、p-Akt蛋白表达均降低(P<0.05),且伊马替尼组<ESC高剂量组<ESC低剂量组(F/P=153.452/0.000、71.294/0.000、182.524/0.000、18.265/0.000、79.628/0.000、9.628/0.000、16.572/0.000、43.827/0.000);4组CAL27细胞Akt蛋白表达水平比较,差异无统计学意义(P>0.05)。结论ESC可能通过抑制PDGFB/PDGFRβ信号通路的激活,从而达到抑制CAL27细胞增殖、迁移和侵袭的目的。

Objective To observe the effects of extract of semen coicix(ESC)on the proliferation,migration and invasion of oral squamous cell carcinoma cells via the platelet-derived growth factor B(PDGFB)/platelet-derived growth factor receptorβ(PDGFRβ)signaling pathway.Methods Experiments will be conducted in the Laboratory of Gansu Provincial People's Hospital from June to August 2020.Human oral squamous cell carcinoma CAL27 cells were cultured in vitro,with control group(no treatment),ESC low-dose group(10μmol/L),ESC high-dose group(20μmol/L)and imatinib group(10μmol/L),after 24 hours of culture,the cell proliferation rate,migration ability,and invasion ability were detected.At the same time,Western blotting was used to detect PDGFB,PDGFRβ,phosphorylated PDGFRβ(p-PDGFRβ),phosphatidylinositol 3 kinase(PI3K),protein kinase B(Akt)and phosphorylated Akt(p-Akt)protein expression levels.Results Compared with the control group,the proliferation rate,migration ability,cell invasion number and PDGFB,PDGFRβ,p-PDGFRβ,PI3K,and p-Akt protein expressions of CAL27 cells in the ESC low-dose group,ESC high-dose group and imatinib group decreased(P<0.05),and imatinib group<ESC high dose group<ESC low dose group(F/P=153.452/0.000,71.294/0.000,182.524/0.000,18.265/0.000,79.628/0.000,9.628/0.000,16.572/0.000,43.827/0.000).There was no statistically significant difference in Akt protein expression level of CAL27 cells in the 4 groups(P>0.05).Conclusion ESC may inhibit the activation of the PDGFB/PDGFRβsignaling pathway to inhibit the proliferation,migration and invasion of CAL27 cells.