CARD11在非特殊型浸润性乳腺癌组织中的表达及临床意义

Expression of CARD11 in non-specific invasive breast carcinoma tissues and its clinical significance

目的探讨CARD11蛋白在非特殊型浸润性乳腺癌(IBC-NST)组织中的表达及其与预后的关系。方法收集2013年1月至2018年12月120例IBC-NST患者的临床病理资料,选取同期60例导管内癌组织和30例癌旁组织作为对照,采用En Vision两步法对CARD11进行免疫组织化学染色,分析CARD11表达与IBC-NST临床病理特征及无进展生存时间(PFS)的关系。结果CARD11蛋白在IBC-NST组织中的阳性表达率为68.3%(83/120),高于导管内癌组织的40.0%(24/60)和癌旁组织的10.0%(3/30),差异有统计学意义(P<0.001)。CARD11的表达与肿瘤最大径、组织学分级、TNM分期、脉管侵犯、淋巴结转移和分子分型有关(P<0.05)。CARD11阳性表达组IBC-NST患者的1、3、5年无进展生存率分别为93.5%、60.4%、10.1%,CARD11阴性表达组分别为98.3%、70.1%、39.2%,两组比较差异有统计学意义(P=0.027)。单因素分析显示,IBC-NST患者的PFS与肿瘤最大径、组织学分级、TNM分期、淋巴结转移、分子分型和CARD11蛋白表达有关(P<0.05)。Cox多因素分析显示,TNM分期、淋巴结转移和CARD11蛋白表达为影响PFS的独立因素(P<0.05)。结论CARD11蛋白在IBC-NST组织中表达升高,且是影响预后的独立因素,阳性表达患者预后不良。

Objective To investigate the expression of CARD11 in invasive breast carcinoma of no specific type(IBC-NST)and its relationship with prognosis.Methods The clinicopathological data of 120 patients with IBC-NST from January 2013 to December 2018 were collected.Sixty cases of intraductal carcinoma tissues and 30 cases of adjacent breast tissues were selected as control.The CARD11 was stained by immunohistochemistry using EnVision two-step method,and the correlation between CARD11 expression and the clinicopathological features and progression-free survival(PFS)of the patients was analyzed.Results The positive rates of CARD11 protein were 68.3%(83/120),40.0%(24/60)and 10.0%(3/30)in IBC-NST,intraductal carcinoma and paracancer tissues,respectively(P<0.001).The expression of CARD11 was significantly correlated with tumor maximum meridian,histological grading,TNM stage,vascular invasion,lymph node metastasis and molecular typing(P<0.05).The 1-,3-,and 5-year progression-free survival rates of IBC-NST patients in CARD11 positive group were 93.5%,60.4%and 10.1%,respectively,while those of CARD11 negative group were 98.3%,70.1%and 39.2%,respectively.There was significant difference between the two groups(P=0.027).Univariate analysis showed that PFS of IBC-NST patients was associated with tumor maximum meridian,histological grade,TNM stage,lymph node metastasis,molecular typing and CARD11 protein expression(P<0.05).Multivariate analysis showed that TNM stage,lymph node metastasis and CARD11 protein expression were independent factors influencing PFS(P<0.05).Conclusion The expression of CARD11 raised in IBC-NST tissues,and it is an independent factor affecting the prognosis of IBC-NST.Paitents with positive CARD11 expression has poor prognosis.