不同组织来源生物补片降解性能的比较研究

Comparative study on the in vivo and in vitro degradation process of biological grafts derived from different tissues

目的比较3种不同组织来源生物补片的体内外降解性能。方法采用Ⅰ型胶原酶对来源于基底膜(BM)与小肠黏膜下层(SIS)复合细胞外基质、单纯小肠黏膜下层、戊二醛化学交联心包(PC)三种生物补片进行体外降解试验,确定各材料的降解率;采用各材料修补大鼠腹壁肌部分层次缺损评估3种补片的降解和组织修复情况。结果体外降解试验中,酶溶液作用120 h后,非交联的BM/SIS复合补片和SIS补片完全降解,PC补片的降解率仅为4.3%±1.9%。体内降解试验中,术后2个月,大体观和组织病理切片染色结果证实BM/SIS复合补片完全降解,组织重塑,再生胶原有序;SIS补片完全降解,再生胶原有序性较差;术后12个月,PC补片未见明显降解,未见细胞长入补片中央区。结论虽然BM/SIS复合补片的体内外完全降解时间均较短,但其可以实现组织的良好快速重塑,提示BM/SIS复合补片是一种降解与再生同步的组织修复材料。

Objective Degradation behaviors of biological grafts derived from different tissue were investigated by taking the degradation experiments in vivo and in vitro respectively.Methods In vitro,solutions of collagenase type I was used to mimic the degradation of biological grafts including the basement membrane(BM)/small intestine submucosa(SIS)composite extracellular matrix(ECM)graft,SIS and chemical cross-linking pericardium(PC).Wistar mice were used as abdominal degradation model in vivo to evaluate the degradation process and therapeutic effect of biological grafts derived from different tissue.Results Results showed that the non-crosslinked BM/SIS and SIS matrix were totally degraded in 120 h for collagenase I in vitro corresponding the degradation rate of the PC patch was only 4.3%±1.9%.BM/SIS matrix was totally degraded in 2 months in vivo,the samples replaced with dense and well-organized collagen fibers.At 2 months post-surgery,SIS also was degraded.While SIS had reconstructed tissue defects with novel-organized collagen fibers.During the experimental period,no degradation was observed and fibrous encapsulations were formed in pericardium repaired samples at 12 months post-surgery.Conclusion Results demonstrated the processes of degradation and tissue repair were matched well of BM composite graft with a better degradation rate and tissue regeneration compared with SIS and pericardium.