不同来源复水液对冻干PRP活化和释放生长因子影响的比较

Comparison of the Effect of Rehydration Solutions Prepared in Different Ingredients on Freeze-dried Plateletrich Plasma Activation and Release of Growth Factors

目的比较不同复水液对冻干富血小板血浆(PRP)活化和释放生长因子含量的影响。方法实验按复水液的不同,分为贫血小板血浆(PPP)(对照组)、生理盐水(实验组1)、超纯水(实验组2)和磷酸缓冲盐溶液(PBS缓冲液)(实验组3)4个组。用四种复水液对冻干PRP复水后,用ELISA法测定其CD63、P-选择素(P-Selectin)、转化生长因子-β1(TGF-β1)、血管内皮生长因子(VEGF)、血小板衍生生长因子BB(PDGF-BB)、成纤维细胞生长因子(bFGF)含量。结果生理盐水组、超纯水组和PBS缓冲液组的P-Selectin和CD63的数值稍高于PPP组,但差异无统计学意义(P>0.05)。三种复水液的生长因子含量均低于PPP组,除IGF-I含量差异有统计学意义外,其余四种生长因子的含量与PPP组相比差异均无统计学意义(P>0.05)。结论生理盐水易得、来源安全,作为复水液对冻干后的血小板活化影响小,生长因子浓度等与PPP复水液复水效果相比无较大差异,可作为冻干PRP的常规复水液。

Objective To evaluate the efficacy of rehydration solutions prepared in different ingredients on freezedried platelet-rich plasma(FD-PRP)activation and release of growth factors.Methods The experiments were divided into four groups:control group,platelet-poor plasma(PPP);group 1,saline solution;group 2,ultrapure water;group 3,phosphate-buffered saline(PBS)buffer according to the difference between rehydration solutions.After rehydrated FD-PRP with four rehydration solutions,the contents of CD63,P-Selectin,transforming growth factor-beta 1(TGF-β1),vascular endothelial growth factor(VEGF),platelet-derived growth factor-BB(PDGF-BB)and basic fibroblast growth factor(bFGF)were determined by ELISA.Results While P-Selectin and CD63 expression in saline group,ultrapure water group and PBS buffer group were slightly higher than those in PPP group,there was no significant difference.The levels of growth factors in other three rehydration solutions were lower than those in PPP group.There was no significant difference in other four growth factors levels between other three groups and PPP group,except for the statistically significant difference in insulin-like growth factor-I(IGF-I).Conclusion Normal saline is widely available and safe.As a rehydration solution,it had little impact on freeze-dried platelet activation.The levels of growth factors had no significant difference compared to PPP rehydration solution.It can be used as a routine rehydration solution for FD-PRP.