合肥地区无偿献血人群CD36抗原缺失的调查

Investigation of CD36 Antigen Deficiency in Unpaid Blood Donors in Hefei

目的研究合肥地区无偿献血人群CD36抗原的缺失频率。方法随机留取合肥市中心血站2020年3月~2020年7月血小板无偿捐献者血样973例,流式细胞术检测CD36抗原。EDTA抗凝全血离心制备富血小板血浆,进行血小板CD36抗原检测,下层细胞经红细胞裂解液处理后,进行单核细胞CD36抗原检测。结果973例血小板无偿捐献者中检出CD36抗原缺失者16例,缺失频率为1.64%;其中Ⅰ型缺失2例,Ⅱ型缺失14例,缺失频率分别为0.20%和1.44%。结论合肥地区无偿献血人群存在一定的CD36抗原缺失。

Objective To investigate the frequency of CD36 antigen deficiency in blood donors in Hefei.Methods A total of 973 blood samples of healthy platelet donors were randomly collected in Hefei blood center from March 2020 to July 2020.Flow cytometry was used for CD36 antigen detection.Platelet-rich plasma was prepared for detection CD36 antigen on platelets,and the residual cells were treated with erythrocyte lysate for CD36 antigen on monocytes.Results Among 973 platelet donors,16 donors were found platelet CD36 antigen deficiency,of which 2 were type I CD36 deficiency,14 were type II CD36 deficiency with the frequency 0.21%and 1.44%respectively.Conclusion The blood donors population in Hefei has certain CD36 antigen deficiency.