细胞感受器初级纤毛在小鼠牙发育中的分布特征

Distribution of primary cilia in tooth development of mouse

目的:观察并总结初级纤毛在牙发育中的分布特征,揭示初级纤毛与牙发育的相关性。方法:进行小鼠牙胚组织切片苏木素-伊红(HE)染色和免疫荧光染色,观察初级纤毛在小鼠牙胚发育的蕾状期、帽状期、钟状期以及出生后细胞外基质分泌时期的分布范围和特点。结果:初级纤毛在蕾状期已广泛分布于牙胚上皮和间充质组织,但在上皮基底膜层几乎没有初级纤毛的分布;帽状期和钟状期的内釉上皮中含有初级纤毛的细胞逐渐增多;1周小鼠牙胚中,分泌期的成釉细胞全部具有初级纤毛,且这些细胞中初级纤毛的位置从胚胎期的上皮组织侧转换至基质分泌侧,但同期成牙本质细胞较少初级纤毛分布。结论:初级纤毛在牙胚发育不同时期具有不同的分布特点;初级纤毛在成釉细胞中的出现与位置分布规律,与成釉细胞的分化状态密切相关;初级纤毛动态参与了小鼠牙发育的整个过程。

Objective: To explore the distribution characteristics of primary cilia in tooth development and further reveal the correlation between primary cilia and tooth development. Methods: Hematoxylin-eosin(HE)staining and immunofluorescence staining of histological sections were used to observe the distribution characteristics of primary cilia in the bud,cap,and bell stages of mouse tooth germ development,as well as the matrix secretion period after birth. Results: The primary cilia were widely distributed in the bud stage,while rarely observed in the basement membrane of the epithelium;cells with primary cilia in inner enamel epithelium gradually increased at the cap and bell stages;in one-week mouse tooth germs,all ameloblasts in the secretory phase had primary cilia,and the location of primary cilia in these cells was switched to the matrix secretion side,while there are fewer primary cilia in the odontoblasts during the same period. Conclusions: Primary cilia have different distribution characteristics at different stages of tooth germ development;the appearance and location of primary cilia in ameloblasts are closely related to the differentiation of ameloblasts;primary cilia are dynamically involved in the whole process of mouse tooth development.