甲状腺乳头状癌中PDK1、ZEB1及P-cadherin的表达和临床意义

Expression and Clinical Significance of PDK1,ZEB1 and P-cadherin in Papillary Thyroid Carcinoma

目的:探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中3-磷酸肌醇依赖性蛋白激酶1(PDK1)、E盒结合锌指蛋白1(ZEB1)和P-钙黏蛋白(P-cadherin)的表达情况和临床意义。方法:选取206例PTC和45例癌旁甲状腺标本,采用免疫组化检测PDK1、ZEB1和P-cadherin蛋白表达,并分析三个指标蛋白表达水平和临床病理各参数的关系。结果:PTC组织中PDK1、ZEB1阳性表达率分别为76.7%、72.3%,高于癌旁甲状腺组织的26.7%、28.9%(P<0.05)。P-cadherin在PTC组织中和癌旁甲状腺中的阳性表达率分别为22.3%、88.9%,两者比较差异有统计学意义(P<0.05)。PDK1、ZEB1及P-cadherin表达与TNM分期、腺体外侵及颈淋巴结转移有关(P<0.05);Spearman等级检验分析,PTC组织中PDK1和ZEB1蛋白表达呈正相关(r_(s)=0.476,P=0.031),PDK1和P-cadherin蛋白表达呈负相关(r_(s)=-0.419,P=0.029),ZEB1和P-cadherin蛋白表达呈显著负相关(r_(s)=-0.586,P=0.019)。结论:PTC中PDK1、ZEB1及P-cadherin蛋白的异常表达与PTC侵袭和转移密切相关,PDK1、ZEB1可能对PTC的诊断和治疗具有重要作用。

Objective:To explore the expression and clinical significance of 3-phosphoinositide dependent protein kinase 1(PDK1),zinc finger E-box-binding protein 1(ZEB1)and P-cadherin protein in papillary thyroid carcinoma.Method:A total of 206 cases of PTC and 45 cases of adjacent thyroid specimens were selected,the expressions of PDK1,ZEB1 and P-cadherin were detected by immunohistochemistry,the relationship between three indicaters protein expression and clinicopathological parameters was analyzed.Result:The positive expression rates of PDK1 and ZEB1 protein in PTC were 76.7%and 72.3%,they were significantly higher than 26.7%and 28.9%in paracancerous normal thyroid tissues(P<0.05).The positive expression rates of P-cadherin in PTC and paracancerous normal thyroid tissues were 22.3%,88.9%,the difference was statistically significant(P<0.05).The differences of the expression of PDK1,ZEB1 and P-cadherin protein in PTC of TNM stage,extrathyroidal invasion and lymph node metastasis were all significant(P<0.05).Spearman rank test showed significantly positive correlation was found between PDK1 and ZEB1(r_(s)=0.476,P=0.031),there was negative correlation between PDK1 and P-cadherin(r_(s)=-0.419,P=0.029),the similar negative correlation was between ZEB1 and P-cadherin(r_(s)=-0.586,P=0.019).Conclusion:The abnormal expression of PDK1,ZEB1 and P-cadherin protein in PTC is closely related to the invasion and metastasis of PTC,PDK1 and ZEB1 may have an important role in the diagnosis and theraphy of PTC.